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Applied and Environmental Microbiology, September 2000, p. 4136-4138, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Starvation Alters the Apparent Half-Saturation Constant for Methane in the Type II Methanotroph Methylocystis Strain LR1

Peter F. Dunfield* and Ralf Conrad

Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch Strasse, 35043 Marburg, Germany

Received 5 May 2000/Accepted 30 June 2000

When cells of a type II methanotrophic bacterium (Methylocystis strain LR1) were starved of methane, both the Km(app) and the Vmax(app) for methane decreased. The specific affinity (aos) remained nearly constant. Therefore, the decreased Km(app) in starved cells was probably not an adjustment to better utilize low-methane concentrations.

* Corresponding author. Mailing address: Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch Strasse, 35043 Marburg, Germany. Phone: (49) 6421-178-733. Fax: (49) 6421-178-809. E-mail: dunfield{at}mailer.uni-marburg.de.

Applied and Environmental Microbiology, September 2000, p. 4136-4138, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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