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Applied and Environmental Microbiology, September 2000, p. 4149-4151, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Determination of the Sensitivity of a Rapid Escherichia coli O157:H7 Assay for Testing 375-Gram Composite Samples

Wan-Ling Tsai,1,2,dagger Cynthia E. Miller,1 and Edward R. Richter1,2,*

Richter International, Inc., Columbus, Ohio 43201,1 and Department of Food Science and Technology, The Ohio State University, Columbus, Ohio 432102

Received 14 October 1999/Accepted 16 June 2000

Both 25-g single-size ground beef samples and 375-g composite ground beef samples were tested by a method combining an immunomagnetic separation (IMS) technique with a sandwich enzyme-linked immunosorbent assay (ELISA) system (IMS-ELISA). The results demonstrated that IMS-ELISA could detect the target, Escherichia coli O157:H7, at the level of 10-1 CFU/g of sample in either the 25- or 375-g sample size.

* Corresponding author. Mailing address: Richter International, Inc., 505 King Ave., Columbus, OH 43201. Phone: (614) 424-3430. Fax: (614) 424-3742. E-mail: ERichter{at}RichterInternational.com.

dagger Present address: Office of Public Affairs and Technology Transfer, Academia Sinica, Taipei, Taiwan, Republic of China.

Applied and Environmental Microbiology, September 2000, p. 4149-4151, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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