This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ozawa, K.
Right arrow Articles by Akutsu, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ozawa, K.
Right arrow Articles by Akutsu, H.
Agricola
Right arrow Articles by Ozawa, K.
Right arrow Articles by Akutsu, H.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 2000, p. 4168-4171, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Expression of a Tetraheme Protein, Desulfovibrio vulgaris Miyazaki F Cytochrome c3, in Shewanella oneidensis MR-1

Kiyoshi Ozawa,1 Alexandre I. Tsapin,2 Kenneth H. Nealson,2,* Michael A. Cusanovich,3 and Hideo Akutsu1

Department of Chemistry and Biotechnology, Faculty of Engineering, Yokohama National University, Hodogaya-ku, Yokohama 240-8501, Japan1; Jet Propulsion Laboratory, California Institute of Technology, Pasadena, California 911092; and Department of Biochemistry, University of Arizona, Tucson, Arizona 857213

Received 9 March 2000/Accepted 6 June 2000

Cytochrome c3 from Desulfovibrio vulgaris Miyazaki F was successfully expressed in the facultative aerobe Shewanella oneidensis MR-1 under anaerobic, microaerophilic, and aerobic conditions, with yields of 0.3 to 0.5 mg of cytochrome/g of cells. A derivative of the broad-host-range plasmid pRK415 containing the cytochrome c3 gene from D. vulgaris Miyazaki F was used for transformation of S. oneidensis MR-1, resulting in the production of protein product that was indistinguishable from that produced by D. vulgaris Miyazaki F, except for the presence of one extra alanine residue at the N terminus.

* Corresponding author. Mailing address: California Institute of Technology, Jet Propulsion Laboratory, Mail Stop 183-301, 4800 Oak Grove Dr., Pasadena, CA 91109. Phone: (818) 354-9219. Fax: (818) 393-4445. E-mail: knealson{at}jpl.nasa.gov.

Applied and Environmental Microbiology, September 2000, p. 4168-4171, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Takayama, Y., Shen, Y., Akutsu, H. (2007). Process of Maturation of Tetraheme Cytochrome c3 in a Shewanella Expression System. J Biochem 141: 121-126 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»