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Applied and Environmental Microbiology, January 2001, p. 453-458, Vol. 67, No. 1
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.1.453-458.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Genetic and Molecular Organization of the Alkylbenzene Catabolism Operon in the Psychrotrophic Strain Pseudomonas putida 01G3

P. A. Chablain,1 A. L. Zgoda,2 C.-O. Sarde,2 and N. Truffaut1,*

Laboratoire de Génétique Microbienne1 and Laboratoire de Technologie Enzymatique,2 UMR 6022 CNRS, Université de Technologie de Compiègne, 60205 Compiègne cedex, France

Received 24 July 2000/Accepted 5 October 2000

The 11-kb sequence encompassing the alkylbenzene upper pathway in Pseudomonas putida 01G3, a psychrotrophic strain able to degrade alkylbenzenes at low temperatures, was characterized. Together with a potential regulator (EbdR), six putative enzymes (EbdAaAbAcAdBC) were identified, and they exhibited highly significant similarities with enzymes implicated in the equivalent pathway in P. putida RE204. ebd genes appeared to be preferentially induced by ethylbenzene. Multiple-alignment data and growth rate measurements led us to classify 01G3 and closely related strains in two groups with distinct substrate specificities. Close to identified genes, remnants of IS5-like elements provided insight into the evolution of catabolic sequences through rearrangements from a less complex ancestral cluster.

* Corresponding author. Mailing address: Laboratoire de Génétique Microbienne, UMR 6022 CNRS, Université de Technologie de Compiègne, B.P. 20529, 60205 Compiègne cedex, France. Phone: 33 3 44 23 44 52. Fax: 33 3 44 20 48 13. E-mail: nicole.truffaut{at}utc.fr.

Applied and Environmental Microbiology, January 2001, p. 453-458, Vol. 67, No. 1
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.1.453-458.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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