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Applied and Environmental Microbiology, January 2001, p. 473-474, Vol. 67, No. 1
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.1.473-474.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Pigment Production by Streptococcus agalactiae in Quasi-Defined Media

Manuel Rosa-Fraile,1,* Antonio Sampedro,1 Javier Rodríguez-Granger,1 Maria Luisa García-Peña,1 Alfonso Ruiz-Bravo,2 and Ali Haïdour3

Microbiology Service, Virgen de las Nieves Hospital, 18014 Granada,1 and Department of Microbiology,2 and Centro de Instrumentación Cientifica,3 University of Granada, 18071 Granada, Spain

Received 24 July 2000/Accepted 28 October 2000

A quasi-defined medium that supports the growth of Streptococcus agalactiae as pigmented colonies has been developed. The medium contains starch, a peptic digest of albumin, amino acids, nucleosides, vitamins, and salts. The presence of free cysteine, which could be replaced with other sulphur-containing compounds and to a lesser degree by reducing agents, was required for pigment formation.

* Corresponding author. Mailing address: Servicio de Microbiologia, Hospital Virgen de las Nieves, 18014 Granada, Spain. Phone: 34-958-241109. Fax: 34-958-241282. E-mail: delarosa{at}cica.es.

Applied and Environmental Microbiology, January 2001, p. 473-474, Vol. 67, No. 1
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.1.473-474.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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Copyright © 2001 by the American Society for Microbiology. All rights reserved.