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Applied and Environmental Microbiology, January 2001, p. 481-483, Vol. 67, No. 1
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.1.481-483.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Effect of Introns and AT-Rich Sequences on Expression of the Bacterial Hygromycin B Resistance Gene in the Basidiomycete Schizophyllum commune

Karin Scholtmeijer,1,* Han A. B. Wösten,1 Jan Springer,2 and Joseph G. H. Wessels1

Molecular Plant Biology Laboratory, Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren,1 and Department of Industrial Microbiology, ATO-DLO, 6700 AA Wageningen,2 The Netherlands

Received 23 May 2000/Accepted 30 October 2000

Previously, it was shown that introns are required for efficient mRNA accumulation in Schizophyllum commune and that the presence of AT-rich sequences in the coding region of genes can result in truncation of transcripts in this homobasidiomycete. Here we show that intron-dependent mRNA accumulation and truncation of transcripts are two independent events that both affect expression of the bacterial hygromycin B resistance gene in S. commune.

* Corresponding author. Present address: BioMaDe Technology, Nijenborgh 4, 9747 AG Groningen, The Netherlands. Phone: 31-50-3635246. Fax: 31-50-3634429. E-mail: K.Scholtmeijer{at}chem.rug.nl.

Applied and Environmental Microbiology, January 2001, p. 481-483, Vol. 67, No. 1
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.1.481-483.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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