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Applied and Environmental Microbiology, January 2001, p. 484-489, Vol. 67, No. 1
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.1.484-489.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Prevalence and Characteristics of Shiga Toxin-Producing Escherichia coli from Healthy Cattle in Japan

Hideki Kobayashi,1,2,* Jun Shimada,3 Muneo Nakazawa,1 Tetsuo Morozumi,1 Tarja Pohjanvirta,2 Sinikka Pelkonen,2 and Koshi Yamamoto1

National Institute of Animal Health, 3-1-1, Kannondai, Tsukuba 305-0856,1 and Chiba Prefectural Institute of Animal Health, 497, Iwatomi, Sakura 285-0072,3 Japan, and National Veterinary and Food Research Institute, Kuopio Regional Laboratory, FIN-70701, Kuopio, Finland2

Received 5 June 2000/Accepted 20 October 2000

The prevalence of Shiga toxin-producing Escherichia coli (STEC) in Japan was examined by using stool samples from 87 calves, 88 heifers, and 183 cows on 78 farms. As determined by screening with stx-PCR, the prevalence was 46% in calves, 66% in heifers, and 69% in cows; as determined by nested stx-PCR, the prevalence was 100% in all animal groups. Of the 962 isolates picked by colony stx hybridization, 92 isolates from 54 farms were characterized to determine their O serogroups, virulence factor genes, and antimicrobial resistance. Of these 92 isolates, 74 (80%) could be classified into O serogroups; 50% of these 74 isolates belonged to O serogroups O8, O26, O84, O113, and O116 and 1 isolate belonged to O serogroup O157. Locus of enterocyte effacement genes were detected in 24% of the isolates, and enterohemorrhagic E. coli (EHEC) hlyA genes were detected in 72% of the isolates. Neither the bundle-forming pilus gene nor the enteropathogenic E. coli adherence factor plasmid was found. STEC strains with characteristics typical of isolates from human EHEC infections, which were regarded as potential EHEC strains, were present on 11.5% of the farms.

* Corresponding author. Mailing address: National Institute of Animal Health, 3-1-1, Kannondai, Tsukuba 305-0856, Japan. Phone: 81-298-387739. Fax: 81-298-387740. E-mail: reptile{at}niah.affrc.go.jp.

Applied and Environmental Microbiology, January 2001, p. 484-489, Vol. 67, No. 1
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.1.484-489.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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