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Applied and Environmental Microbiology, October 2001, p. 4407-4413, Vol. 67, No. 10
Research Group of Industrial Microbiology,
Fermentation Technology and Downstream Processing (IMDO), Department of
Applied Biological Sciences, Vrije Universiteit Brussel (VUB), B-1050
Brussels, Belgium
Received 2 April 2001/Accepted 8 July 2001
Although commercial MRS broth has been designed to allow excellent
growth of lactobacilli, most of these bacteria are still subjected to a
self-inhibiting process. The most likely explanation is the
accumulation of lactic acid or other toxic end products and the
depletion of nutrients. In this study, the self-inhibition of
Lactobacillus sakei CTC 494 was analyzed in a kinetic
way, and a nutrient depletion model was set up to describe the growth inhibition process. This simple model has considerable advantages compared to commonly used descriptive models such as the logistic growth equation. It offers a better fit and a more realistic
description of the growth data by taking into account both growth
inhibition due to lactic acid production and changes in growth rates
due to nutrient depletion. Depending on the fermentation conditions, in
MRS broth there appears to be a strong decrease of the specific growth
rate over time. Some undefined compounds present in the complex
nitrogen source of MRS broth appear to be of crucial importance because
of their limited availability. Moreover, nutrient availability affects
bacteriocin production through its effect on cell growth as well as on
the bacteriocin production per cell. A plateau value for the
bacteriocin production by L. sakei CTC 494 was observed.
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.10.4407-4413.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Growth of the Bacteriocin-Producing
Lactobacillus sakei Strain CTC 494 in MRS Broth Is
Strongly Reduced Due to Nutrient Exhaustion: a Nutrient
Depletion Model for the Growth of Lactic Acid Bacteria
*
Corresponding author. Mailing address: Research Group
of Industrial Microbiology, Fermentation Technology and Downstream
Processing (IMDO), Department of Applied Biological Sciences, Vrije
Universiteit Brussel (VUB), Pleinlaan 2, B-1050 Brussels, Belgium.
Phone: 32-2-6293245. Fax: 32-2-6292720. E-mail:
ldvuyst{at}vub.ac.be.
Applied and Environmental Microbiology, October 2001, p. 4407-4413, Vol. 67, No. 10
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.10.4407-4413.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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