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Applied and Environmental Microbiology, October 2001, p. 4440-4447, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4440-4447.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Effect of Selenite on Growth and Protein Synthesis in the Phototrophic Bacterium Rhodobacter sphaeroides

Magali Bebien,1 Jean-Paul Chauvin,2 Jean-Marc Adriano,1 Sandrine Grosse,1 and André Verméglio1,*

CEA/Cadarache-DSV-DEVM-Laboratoire de Bioénergétique Cellulaire, 13108 Saint-Paul-lez-Durance,1 and Institut de Biologie du Développement de Marseille-Faculté des Sciences de Luminy-UMR 6549-13288 Marseille,2 France

Received 26 February 2001/Accepted 17 July 2001

The effect of selenite on the growth rate and protein synthesis has been investigated in Rhodobacter sphaeroides. This photosynthetic bacterium efficiently reduces selenite with intracellular accumulation under both dark aerobic and anaerobic photosynthetic conditions. Addition of 1 mM selenite under these two growth conditions does not affect the final cell density, although a marked slowdown in growth rate is observed under aerobic growth. The proteome analysis of selenite response by two-dimensional gel electrophoresis shows an enhanced synthesis of some chaperones, an elongation factor, and enzymes associated to oxidative stress. The induction of these antioxidant proteins confirms that the major toxic effect of selenite is the formation of reactive oxygen species during its metabolism. In addition, we show that one mutant unable to precipitate selenite, selected from a transposon library, is affected in the smoK gene. This encodes a constituent of a putative ABC transporter implicated in the uptake of polyols. This mutant is less sensitive to selenite and does not express stress proteins identified in the wild type in response to selenite. This suggests that the entry of selenite into the cytoplasm is mediated by a polyol transporter in R. sphaeroides.

* Corresponding author. Mailing address: Département d'Écophysiologie Végétale et Microbiologie, CEA/Cadarache-DSV-DEVM-Laboratoire de Bioénergétique Cellulaire, 13108 Saint-Paul-lez- Durance Cedex, France. Phone: 33-442254630. Fax: 33-442254701. E-mail: avermeglio{at}cea.fr.

Applied and Environmental Microbiology, October 2001, p. 4440-4447, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4440-4447.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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