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Applied and Environmental Microbiology, October 2001, p. 4828-4833, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4828-4833.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Streptococcus suis Serotypes Characterized by Analysis of Chaperonin 60 Gene Sequences

Ronald Brousseau,1,* Janet E. Hill,2 Gabrielle Préfontaine,1 Swee-Han Goh,3 Josée Harel,4 and Sean M. Hemmingsen2

Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec H4P 2R2,1 Plant Biotechnology Institute, National Research Council of Canada, Saskatoon, Saskatchewan,2 Department of Pathology and Laboratory Medicine, University of British Columbia, and B.C. Centre for Disease Control Society, Vancouver, British Columbia,3 and Groupe de recherche sur les Maladies infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, CP 5000, St-Hyacinthe, Québec, Canada4

Received 6 April 2001/Accepted 18 July 2001

Streptococcus suis is an important pathogen of swine which occasionally infects humans as well. There are 35 serotypes known for this organism, and it would be desirable to develop rapid methods methods to identify and differentiate the strains of this species. To that effect, partial chaperonin 60 gene sequences were determined for the 35 serotype reference strains of S. suis. Analysis of a pairwise distance matrix showed that the distances ranged from 0 to 0.275 when values were calculated by the maximum-likelihood method. For five of the strains the distances from serotype 1 were greater than 0.1, and for two of these strains the distances were were more than 0.25, suggesting that they belong to a different species. Most of the nucleotide differences were silent; alignment of protein sequences showed that there were only 11 distinct sequences for the 35 strains under study. The chaperonin 60 gene phylogenetic tree was similar to the previously published tree based on 16S rRNA sequences, and it was also observed that strains with identical chaperonin 60 gene sequences tended to have identical 16S rRNA sequences. The chaperonin 60 gene sequences provided a higher level of discrimination between serotypes than the 16S RNA sequences provided and could form the basis for a diagnostic protocol.

* Corresponding author. Mailing address: Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Avenue, Montreal, Quebec H4P 2R2, Canada. Phone: (514) 496-6152. Fax: (514) 496-6213. E-mail: Roland.Brousseau{at}nrc.ca.

Applied and Environmental Microbiology, October 2001, p. 4828-4833, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4828-4833.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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