This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fente, C. A.
Right arrow Articles by Cepeda, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fente, C. A.
Right arrow Articles by Cepeda, A.
Agricola
Right arrow Articles by Fente, C. A.
Right arrow Articles by Cepeda, A.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 2001, p. 4858-4862, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4858-4862.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

New Additive for Culture Media for Rapid Identification of Aflatoxin-Producing Aspergillus Strains

C. A. Fente,1,* J. Jaimez Ordaz,1 B. I. Vázquez,2 C. M. Franco,1 and A. Cepeda1

Laboratorio de Higiene e Inspección de Alimentos, Departamento de Química Analítica, Nutrición y Bromatología, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27002 Lugo,1 and Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Ciencias Experimentales y de la Salud: Veterinaria, Universidad Cardenal Herrera-CEU, 46113 Moncada, Valencia,2 Spain

Received 7 February 2001/Accepted 16 July 2001

A new reliable, fast, and simple method for the detection of aflatoxigenic Aspergillus strains, consisting of the addition of a cyclodextrin (a methylated beta -cyclodextrin derivative) to common media used for testing mycotoxin production ability, was developed. We propose the use of this compound as an additive for fungal culture media to enhance the natural fluorescence of aflatoxins. The production of aflatoxins coincided with the presence of a bright blue or blue-green fluorescent area surrounding colonies when observed under long-wavelength (365-nm) UV light after 3 days of incubation at 28°C. The presence of aflatoxins was confirmed by extracting the medium with chloroform and examining the extracts by high-pressure liquid chromatography with fluorescence detection.

* Corresponding author. Mailing address: Laboratorio de Higiene e Inspección de Alimentos, Dpto. Química Analítica, Nutrición y Bromatología, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27002-Lugo, Spain. Phone and fax: 34-982254592. E-mail: cfente{at}correo.lugo.usc.es.

Applied and Environmental Microbiology, October 2001, p. 4858-4862, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4858-4862.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Atanda, O. O., Akpan, I., Rati, E. R., Ozoje, M. (2005). Palm Kernel: A Potential Substrate for Rapid Detection of Aflatoxigenic Fungi. Food Science and Technology International 11: 67-74 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»