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Applied and Environmental Microbiology, October 2001, p. 4891-4895, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4891-4895.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Detection of Indigenous Halobacillus Populations in Damaged Ancient Wall Paintings and Building Materials: Molecular Monitoring and Cultivation

Guadalupe Piñar,1,* Cayo Ramos,2,dagger Sabine Rölleke,3 Claudia Schabereiter-Gurtner,1 Dietmar Vybiral,1 Werner Lubitz,1 and Ewald B. M. Denner1

Institute of Microbiology and Genetics, University of Vienna, A-1030 Vienna, Austria1; Institute of Microbiology, Technical University of Denmark, Lyngby, Denmark2; and Genalysis GmbH, D-14943 Luckenwalde, Germany3

Received 6 April 2001/Accepted 16 July 2001

Several moderately halophilic gram-positive, spore-forming bacteria have been isolated by conventional enrichment cultures from damaged medieval wall paintings and building materials. Enrichment and isolation were monitored by denaturing gradient gel electrophoresis and fluorescent in situ hybridization. 16S ribosomal DNA analysis showed that the bacteria are most closely related to Halobacillus litoralis. DNA-DNA reassociation experiments identified the isolates as a population of hitherto unknown Halobacillus species.

* Corresponding author. Mailing address: Institute of Microbiology and Genetics, University of Vienna, Dr. Bohr-Gasse 9, A-1030 Vienna, Austria. Phone: 0043-1-4277 54675 or 0043-1-4277 54632. Fax: 0043-1-4277 54674. E-mail: upe{at}gem.univie.ac.at.

dagger Present address: Department of Genetics, Faculty of Sciences, University of Málaga, 29071-Málaga, Spain.

Applied and Environmental Microbiology, October 2001, p. 4891-4895, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4891-4895.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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