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Applied and Environmental Microbiology, October 2001, p. 4896-4900, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4896-4900.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Assessing the Diversity of Marine Bacterial beta -Glucosidases by Capillary Electrophoresis Zymography†

Jesús M. Arrieta and Gerhard J. Herndl*

Department of Biological Oceanography, Netherlands Institute for Sea Research, 1790 AB Den Burg, Texel, The Netherlands

Received 7 May 2001/Accepted 31 July 2001

We propose a new method for the fast separation and detection of beta -glucosidases in environmental samples. With this approach, beta -glucosidases extracted from bacteria are evidenced by substrate-incorporated capillary electrophoresis (CE zymography) and their kinetic parameters can be determined by repeated injections using different substrate concentrations. Preliminary results obtained with natural bacterial communities from the coastal North Sea suggest that the diversity of beta -glucosidases in the marine environment might be much higher than previously observed.

* Corresponding author. Mailing address: Department of Biological Oceanography, Netherlands Institute for Sea Research (NIOZ), P.O. Box 59, 1790 AB Den Burg, Texel, The Netherlands. Phone: 31-222-369-507. Fax: 31-222-319-674. E-mail: herndl{at}nioz.nl.

dagger This is NIOZ contribution number 3631.

Applied and Environmental Microbiology, October 2001, p. 4896-4900, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4896-4900.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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