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Applied and Environmental Microbiology, October 2001, p. 4901-4907, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4901-4907.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Variation in Resistance to High Hydrostatic Pressure and rpoS Heterogeneity in Natural Isolates of Escherichia coli O157:H7

Marianne Robey,1 Amparo Benito,2 Roger H. Hutson,2 Cristina Pascual,2 Simon F. Park,3 and Bernard M. Mackey2,*

School of Food Biosciences, University of Reading, Whiteknights, Reading RG6 6AP,2 and School of Biological Sciences, University of Surrey, Guildford, Surrey GU2 5XH,3 United Kingdom, and Department of Microbiology-Immunology, Northwestern University, Chicago, Illinois 606111

Received 4 April 2001/Accepted 17 July 2001

Several natural isolates of Escherichia coli O157:H7 have previously been shown to exhibit stationary-phase-dependent variation in their resistance to inactivation by high hydrostatic pressure. In this report we demonstrate that loss of the stationary-phase-inducible sigma factor RpoS resulted in decreased resistance to pressure in E. coli O157:H7 and in a commensal strain. Furthermore, variation in the RpoS activity of the natural isolates of O157:H7 correlated with the pressure resistance of those strains. Heterogeneity was noted in the rpoS alleles of the natural isolates that may explain the differences in RpoS activity. These results are consistent with a role for rpoS in mediating resistance to high hydrostatic pressure in E. coli O157:H7.

* Corresponding author. Mailing address: School of Food Biosciences, University of Reading, P.O. Box 226, Whiteknights, Reading, RG6 6AP, United Kingdom. Phone: 44 1189 357 229. Fax: 44 1189 357 222. E-mail: b.m.mackey{at}reading.ac.uk.

Applied and Environmental Microbiology, October 2001, p. 4901-4907, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4901-4907.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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