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Applied and Environmental Microbiology, October 2001, p. 4919-4921, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4919-4921
Copyright © 2001, American Society for Microbiology. All rights reserved.

Isolation of an Aldehyde Dehydrogenase Involved in the Oxidation of Fluoroacetaldehyde to Fluoroacetate in Streptomyces cattleya

Cormac D. Murphy,1,2 Steven J. Moss,2 and David O'Hagan1,2,*

School of Chemistry, University of St. Andrews, Fife KY16 9ST,1 and Department of Chemistry, University of Durham, Durham DH1 3LE,2 United Kingdom

Received 10 April 2001/Accepted 19 June 2001

Streptomyces cattleya is unusual in that it produces fluoroacetate and 4-fluorothreonine as secondary metabolites. We now report the isolation of an NAD+-dependent fluoroacetaldehyde dehydrogenase from S. cattleya that mediates the oxidation of fluoroacetaldehyde to fluoroacetate. This is the first enzyme to be identified that is directly involved in fluorometabolite biosynthesis. Production of the enzyme begins in late exponential growth and continues into the stationary phase. Measurement of kinetic parameters shows that the enzyme has a high affinity for fluoroacetaldehyde and glycoaldehyde, but not acetaldehyde.

* Corresponding author. Mailing address: School of Chemistry, University of St. Andrews, Purdic Building, North Haugh, St. Andrews, Fife KY16 9ST, United Kingdom. Phone: (44) 1334467176. Fax: (44) 1334463808. E-mail: do1{at}st-andrews.ac.uk.

Applied and Environmental Microbiology, October 2001, p. 4919-4921, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4919-4921
Copyright © 2001, American Society for Microbiology. All rights reserved.




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Copyright © 2001 by the American Society for Microbiology. All rights reserved.