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Applied and Environmental Microbiology, October 2001, p. 4922-4925, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4922-4925.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Isolation of Methyl Parathion-Degrading Strain M6 and Cloning of the Methyl Parathion Hydrolase Gene

Cui Zhongli, Li Shunpeng,* and Fu Guoping

Department of Microbiology, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, People's Republic of China

Received 12 March 2001/Accepted 12 July 2001

A degradative bacterium, M6, was isolated and presumptively identified as Plesiomonas sp. strain M6 was able to hydrolyze methyl parathion to p-nitrophenol. A novel organophosphate hydrolase gene designated mpd was selected from its genomic library prepared by shotgun cloning. The nucleotide sequence of the mpd gene was determined. The gene could be effectively expressed in Esherichia coli.

* Corresponding author. Mailing address: Department of Microbiology, Nanjing Agricultural University, 6 Tongwei Rd., Nanjing, Jiangsu Province 210095, People's Republic of China. Phone and fax: 86 25 4396314. E-mail: lsp{at}njau.edu.cn.

Applied and Environmental Microbiology, October 2001, p. 4922-4925, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4922-4925.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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