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Applied and Environmental Microbiology, October 2001, p. 4926-4929, Vol. 67, No. 10
The Wellcome Trust Centre for the
Epidemiology of Infectious Disease, Department of
Zoology,1 and Department of Clinical
Neurology,7 University of Oxford, and
NERC Centre for Ecology and Hydrology,2
Oxford, United Kingdom; ULBL/CMDT, Institute of Hygiene and
Tropical Medicine, Universidade Nova de Lisboa,3
and CBA/Science Faculty, Lisbon
University,4 Lisbon, Portugal; Institute
of Zoology, Slovak Academy of Sciences, Bratislava,
Slovakia5; and Ministry of Welfare
of the Republic of Latvia, Riga, Latvia6
Received 27 December 2000/Accepted 8 July 2001
The genetic diversity of Borrelia burgdorferi sensu
lato was assessed in individual adult Ixodes ricinus
ticks from Europe by direct PCR amplification of spirochetal DNA
followed by genospecies-specific hybridization. Analysis of mixed
infections in the ticks showed that B.
garinii and B. valaisiana
segregate from B. afzelii. This and
previous findings suggest that host complement interacts with spirochetes in the tick, thereby playing an important role in the
ecology of Lyme borreliosis.
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.10.4926-4929.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Distinct Combinations of Borrelia burgdorferi
Sensu Lato Genospecies Found in Individual Questing Ticks
from Europe
*
Corresponding author. Mailing address: The Wellcome
Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, University of Oxford, Oxford, OX1 3PS, United Kingdom. Phone:
0044 (0) 1865-281547. E-mail:
klaus.kurtenbach{at}ceid.ox.ac.uk.
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