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Applied and Environmental Microbiology, November 2001, p. 5025-5031, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5025-5031.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Glucose Uptake in Clostridium beijerinckii NCIMB 8052 and the Solvent-Hyperproducing Mutant BA101

Jieun Lee and H. P. Blaschek*

Food Microbiology Division, Department of Food Science and Human Nutrition, University of Illinois, Urbana, Illinois 61801

Received 6 March 2001/Accepted 25 June 2001

Glucose uptake and accumulation by Clostridium beijerinckii BA101, a butanol hyperproducing mutant, were examined during various stages of growth. Glucose uptake in C. beijerinckii BA101 was repressed 20% by 2-deoxyglucose and 25% by mannose, while glucose uptake in C. beijerinckii 8052 was repressed 52 and 28% by these sugars, respectively. We confirmed the presence of a phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) associated with cell extracts of C. beijerinckii BA101 by glucose phosphorylation by PEP. The PTS activity associated with C. beijerinckii BA101 was 50% of that observed for C. beijerinckii 8052. C. beijerinckii BA101 also demonstrated lower PTS activity for fructose and glucitol. Glucose phosphorylation by cell extracts derived from both C. beijerinckii BA101 and 8052 was also dependent on the presence of ATP, a finding consistent with the presence of glucokinase activity in C. beijerinckii extracts. ATP-dependent glucose phosphorylation was predominant during the solventogenic stage, when PEP-dependent glucose phosphorylation was dramatically repressed. A nearly twofold-greater ATP-dependent phosphorylation rate was observed for solventogenic stage C. beijerinckii BA101 than for solventogenic stage C. beijerinckii 8052. These results suggest that C. beijerinckii BA101 is defective in PTS activity and that C. beijerinckii BA101 compensates for this defect with enhanced glucokinase activity, resulting in an ability to transport and utilize glucose during the solventogenic stage.

* Corresponding author. Mailing address: Food Microbiology Division, Department of Food Science and Human Nutrition, University of Illinois, 1207 W. Gregory Dr., Urbana, IL 61801. Phone: (217) 333-8224. Fax: (217) 244-2517. E-mail: blaschek{at}uiuc.edu.

Applied and Environmental Microbiology, November 2001, p. 5025-5031, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5025-5031.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Shi, Z., Blaschek, H. P. (2008). Transcriptional Analysis of Clostridium beijerinckii NCIMB 8052 and the Hyper-Butanol-Producing Mutant BA101 during the Shift from Acidogenesis to Solventogenesis. Appl. Environ. Microbiol. 74: 7709-7714 [Abstract] [Full Text]  
  • Lee, J., Mitchell, W. J., Tangney, M., Blaschek, H. P. (2005). Evidence for the Presence of an Alternative Glucose Transport System in Clostridium beijerinckii NCIMB 8052 and the Solvent-Hyperproducing Mutant BA101. Appl. Environ. Microbiol. 71: 3384-3387 [Abstract] [Full Text]  


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