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Applied and Environmental Microbiology, November 2001, p. 5043-5048, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5043-5048.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

High Genetic Variability for Resistance to Bacillus thuringiensis Toxins in a Single Population of Diamondback Moth

Joel González-Cabrera, Salvador Herrero, and Juan Ferré^*

Department of Genetics, Universitat de València, 46100-Burjassot (Valencia), Spain

Received 14 May 2001/Accepted 11 August 2001

The long-term benefit of insecticidal products based on Cry toxins, either in sprays or as transgenic crops, is threatened by the development of resistance by target pests. The models used to predict evolution of resistance to Cry toxins most often are monogenic models in which two alleles are used. Moreover, the high-dose/refuge strategy recommended for implementation with transgenic crops relies on the assumption that the resistance allele is recessive. Using selection experiments, we demonstrated the occurrence in a laboratory colony of diamondback moth of two different genes (either allelic or nonallelic) that confer resistance to Cry1Ab. At the concentration tested, resistance was dominant in one selection line and partially recessive in the other. Resistant insects from the two selection lines also differed in their cross-resistance patterns. The diamondback moth colony was derived from a field population from the Philippines, which originally showed a different resistance phenotype. This is the first time that an insect population has been directly shown to carry more than one gene conferring resistance to the same Cry toxin.

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^* Corresponding author. Mailing address: Departament de Genètica, Facultat de CC. Biològiques, Dr. Moliner 50, 46100-Burjassot (Valencia), Spain. Phone: (34) 96 386 4506. Fax: (34) 96 398 3029. E-mail: Juan.Ferre{at}uv.es.

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Applied and Environmental Microbiology, November 2001, p. 5043-5048, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5043-5048.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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