This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wouters, J. A.
Right arrow Articles by Abee, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wouters, J. A.
Right arrow Articles by Abee, T.
Agricola
Right arrow Articles by Wouters, J. A.
Right arrow Articles by Abee, T.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2001, p. 5171-5178, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5171-5178.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Cold Shock Proteins of Lactococcus lactis MG1363 Are Involved in Cryoprotection and in the Production of Cold-Induced Proteins

Jeroen A. Wouters,1,2,* Hélène Frenkiel,2,3 Willem M. de Vos,2,3 Oscar P. Kuipers,2,dagger and Tjakko Abee1

Laboratory of Food Microbiology, Wageningen University,1 and Wageningen Centre for Food Sciences (WCFS),3 Wageningen, and Microbial Ingredients Section, NIZO Food Research, Ede,2 The Netherlands

Received 27 April 2001/Accepted 28 August 2001

Members of the group of 7-kDa cold-shock proteins (CSPs) are the proteins with the highest level of induction upon cold shock in the lactic acid bacterium Lactococcus lactis MG1363. By using double-crossover recombination, two L. lactis strains were generated in which genes encoding CSPs are disrupted: L. lactis NZ9000Delta AB lacks the tandemly orientated cspA and cspB genes, and NZ9000Delta ABE lacks cspA, cspB, and cspE. Both strains showed no differences in growth at normal and at low temperatures compared to that of the wild-type strain, L. lactis NZ9000. Two-dimensional gel electrophoresis showed that upon disruption of the cspAB genes, the production of remaining CspE at low temperature increased, and upon disruption of cspA, cspB, and cspE, the production of CspD at normal growth temperatures increased. Northern blot analysis showed that control is most likely at the transcriptional level. Furthermore, it was established by a proteomics approach that some (non-7-kDa) cold-induced proteins (CIPs) are not cold induced in the csp-lacking strains, among others the histon-like protein HslA and the signal transduction protein LlrC. This supports earlier observations (J. A. Wouters, M. Mailhes, F. M. Rombouts, W. M. De Vos, O. P. Kuipers, and T. Abee, Appl. Environ. Microbiol. 66:3756-3763, 2000). that the CSPs of L. lactis might be directly involved in the production of some CIPs upon low-temperature exposure. Remarkably, the adaptive response to freezing by prior exposure to 10°C was significantly reduced in strain NZ9000Delta ABE but not in strain NZ9000Delta AB compared to results with wild-type strain NZ9000, indicating a notable involvement of CspE in cryoprotection.

* Corresponding author. Mailing address: Laboratory of Food Microbiology, Wageningen University, Bomenweg 2, 6703 HD Wageningen, The Netherlands. Phone: 31-317-484981. Fax: 31-317-484978. E-mail: jeroen.wouters{at}micro.fdsci.wau.nl.

dagger Present address: Department of Genetics, Groningen Biomolecular Sciences and Biotechnology institute (GBB), University of Groningen, 9750 AA Haren, The Netherlands.

Applied and Environmental Microbiology, November 2001, p. 5171-5178, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5171-5178.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Gagnaire, V., Jardin, J., Jan, G., Lortal, S. (2009). Invited review: Proteomics of milk and bacteria used in fermented dairy products: From qualitative to quantitative advances. J DAIRY SCI 92: 811-825 [Abstract] [Full Text]  
  • Monnet, C., Ulve, V., Sarthou, A.-S., Irlinger, F. (2008). Extraction of RNA from Cheese without Prior Separation of Microbial Cells. Appl. Environ. Microbiol. 74: 5724-5730 [Abstract] [Full Text]  
  • Hufner, E., Markieton, T., Chaillou, S., Crutz-Le Coq, A.-M., Zagorec, M., Hertel, C. (2007). Identification of Lactobacillus sakei Genes Induced during Meat Fermentation and Their Role in Survival and Growth. Appl. Environ. Microbiol. 73: 2522-2531 [Abstract] [Full Text]  
  • O'Driscoll, J., Glynn, F., Fitzgerald, G. F., Sinderen, D. v. (2006). Sequence Analysis of the Lactococcal Plasmid pNP40: a Mobile Replicon for Coping with Environmental Hazards.. J. Bacteriol. 188: 6629-6639 [Abstract] [Full Text]  
  • Wang, Y., Corrieu, G., Beal, C. (2005). Fermentation pH and Temperature Influence the Cryotolerance of Lactobacillus acidophilus RD758. J DAIRY SCI 88: 21-29 [Abstract] [Full Text]  
  • Derzelle, S., Hallet, B., Ferain, T., Delcour, J., Hols, P. (2003). Improved Adaptation to Cold-Shock, Stationary-Phase, and Freezing Stresses in Lactobacillus plantarum Overproducing Cold-Shock Proteins. Appl. Environ. Microbiol. 69: 4285-4290 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»