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Applied and Environmental Microbiology, November 2001, p. 5210-5218, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5210-5218.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Comparison of Cellular and Biomass Specific Activities of Dominant Bacterioplankton Groups in Stratified Waters of the Celtic Sea

Mikhail V. Zubkov,1,* Bernhard M. Fuchs,2 Peter H. Burkill,1 and Rudolf Amann2

Plymouth Marine Laboratory, Plymouth PL1 3DH, United Kingdom,1 and Max Planck Institute for Marine Microbiology, D-28359 Bremen, Germany2

Received 13 June 2001/Accepted 21 August 2001

A flow-sorting technique was developed to determine unperturbed metabolic activities of phylogenetically characterized bacterioplankton groups with incorporation rates of [35S]methionine tracer. According to fluorescence in situ hybridization with rRNA targeted oligonucleotide probes, a clade of alpha -proteobacteria, related to Roseobacter spp., and a Cytophaga-Flavobacterium cluster dominated the different groups. Cytometric characterization revealed both these groups to have high DNA (HNA) content, while the alpha -proteobacteria exhibited high light scatter (hs) and the Cytophaga-Flavobacterium cluster exhibited low light scatter (ls). A third abundant group with low DNA (LNA) content contained cells from a SAR86 cluster of gamma -proteobacteria. Cellular specific activities of the HNA-hs group were 4- and 1.7-fold higher than the activities in the HNA-ls and LNA groups, respectively. However, the higher cellular protein synthesis by the HNA-hs could simply be explained by their maintenance of a larger cellular protein biomass. Similar biomass specific activities of the different groups strongly support the main assumption that underlies the determination of bacterial production: different bacteria in a complex community incorporate amino acids at a rate proportional to their protein synthesis. The fact that the highest growth-specific rates were determined for the smallest cells of the LNA group can explain the dominance of this group in nutrient-limited waters. The metabolic activities of the three groups accounted for almost the total bacterioplankton activity, indicating their key biogeochemical role in the planktonic ecosystem of the Celtic Sea.

* Corresponding author. Mailing address: Plymouth Marine Laboratory, Prospect Place, West Hoe, Plymouth PL1 3DH, United Kingdom. Phone: 44-1752-633422. Fax: 44-1752-633101. E-mail: mvz{at}pml.ac.uk.

Applied and Environmental Microbiology, November 2001, p. 5210-5218, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5210-5218.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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