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Applied and Environmental Microbiology, November 2001, p. 5225-5232, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5225-5232.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Molecular Characterization of a New Abortive Infection System (AbiU) from Lactococcus lactis LL51-1

Gang Dai,1 Ping Su,1,2,* Gwen E. Allison,2 Bruce L. Geller,3 Ping Zhu,2 Woojin S. Kim,1 and Noel W. Dunn1

Department of Biotechnology, The University of New South Wales, Sydney, New South Wales 2052,1 and DSM Food Specialties, Moorebank, New South Wales 1875,2 Australia, and Department of Microbiology, Oregon State University, Corvallis, Oregon 97331-38043

Received 28 February 2001/Accepted 9 August 2001

This study reports on the identification and characterization of a novel abortive infection system, AbiU, from Lactococcus lactis. AbiU confers resistance to phages from the three main industrially relevant lactococcal phage species: c2, 936, and P335. The presence of AbiU reduced the efficiency of plaquing against specific phage from each species as follows: 3.7 × 10-1, 1.0 × 10-2, and 1.0 × 10-1, respectively. abiU involves two open reading frames, abiU1 (1,772 bp) and abiU2 (1,019 bp). Evidence indicates that AbiU1 is responsible for phage resistance and that AbiU2 may downregulate phage resistance against 936 and P335 type phages but not c2 type phage. AbiU appeared to delay transcription of both phage 712 and c2, with the effect being more marked on phage c2.

* Corresponding author. Mailing address: Department of Biotechnology, University of New South Wales, Sydney, NSW 2052, Australia. Phone: 61-2-93853868. Fax: 61-2-93136710. E-mail: p.su{at}unsw.edu.au.

Applied and Environmental Microbiology, November 2001, p. 5225-5232, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5225-5232.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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