AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tag, A. G.
Right arrow Articles by Beremand, M. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tag, A. G.
Right arrow Articles by Beremand, M. N.
Agricola
Right arrow Articles by Tag, A. G.
Right arrow Articles by Beremand, M. N.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2001, p. 5294-5302, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5294-5302.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

A Novel Regulatory Gene, Tri10, Controls Trichothecene Toxin Production and Gene Expression

Andrew G. Tag,1 Gulnara F. Garifullina,1 Andrew W. Peplow,1 Charles Ake Jr.,2 T. D. Phillips,2 Thomas M. Hohn,3 and Marian N. Beremand1,*

Department of Plant Pathology and Microbiology1 and Department of Veterinary Anatomy and Public Health,2 Texas A&M University, College Station, Texas 77843, and Syngenta, Research Triangle Park, North Carolina 277093

Received 24 April 2001/Accepted 20 August 2001

We report here the characterization of Tri10, a novel regulatory gene within the trichothecene gene cluster. Comparison of Tri10 genomic and mRNA sequences revealed that removal of a single 77-bp intron provided a 1,260-bp open reading frame, encoding a 420-amino-acid protein. Disruption of Tri10 in Fusarium sporotrichioides abolished T-2 toxin production and dramatically decreased the transcript accumulation for four trichothecene genes (Tri4, Tri5, Tri6, and Tri101) and an apparent farnesyl pyrophosphate synthetase (Fpps) gene. Conversely, homologous integration of a disruption vector by a single upstream crossover event significantly increased T-2 toxin production and elevated the transcript accumulation of the trichothecene genes and Fpps. Further analysis revealed that disruption of Tri10, and to a greater extent the disruption of Tri6, increased sensitivity to T-2 toxin under certain growth conditions. Although Tri10 is conserved in Fusarium graminearum and Fusarium sambucinum and clearly plays a central role in regulating trichothecene gene expression, it does not show any significant matches to proteins of known or predicted function or to motifs except a single transmembrane domain. We suggest a model in which Tri10 acts upstream of the cluster-encoded transcription factor TRI6 and is necessary for full expression of both the other trichothecene genes and the genes for the primary metabolic pathway that precedes the trichothecene biosynthetic pathway, as well as for wild-type levels of trichothecene self-protection. We further suggest the presence of a regulatory loop where Tri6 is not required for the transcription of Tri10 but is required to limit the expression of Tri10.

* Corresponding author. Mailing address: Department of Plant Pathology and Microbiology, Texas A&M University, 2132 TAMUS, College Station, TX 77843-2132. Phone: (979) 845-4636. Fax: (979) 845-6483. E-mail: m-beremand{at}tamu.edu.

Applied and Environmental Microbiology, November 2001, p. 5294-5302, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5294-5302.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2001 by the American Society for Microbiology. All rights reserved.