This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lovell, C. R.
Right arrow Articles by Bagwell, C. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lovell, C. R.
Right arrow Articles by Bagwell, C. E.
Agricola
Right arrow Articles by Lovell, C. R.
Right arrow Articles by Bagwell, C. E.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2001, p. 5308-5314, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5308-5314.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Recovery and Phylogenetic Analysis of nifH Sequences from Diazotrophic Bacteria Associated with Dead Aboveground Biomass of Spartina alterniflora

Charles R. Lovell,1,* Michael J. Friez,2 John W. Longshore,2 and Christopher E. Bagwell1,dagger

Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208,1 and Molecular Diagnostics Laboratory, Greenwood Genetic Center, Greenwood, South Carolina 296462

Received 4 June 2001/Accepted 21 August 2001

DNA was extracted from dry standing dead Spartina alterniflora stalks as well as dry Spartina wrack from the North Inlet (South Carolina) and Sapelo Island (Georgia) salt marshes. Partial nifH sequences were PCR amplified, the products were separated by denaturing gradient gel electrophoresis (DGGE), and the prominent DGGE bands were sequenced. Most sequences (109 of 121) clustered with those from alpha -Proteobacteria, and 4 were very similar (>99%) to that of Azospirillum brasilense. Seven sequences clustered with those from known gamma -Proteobacteria and five with those from known anaerobic diazotrophs. The diazotroph assemblages associated with dead Spartina biomass in these two salt marshes were very similar, and relatively few major lineages were represented.

* Corresponding author. Mailing address: Department of Biological Sciences, University of South Carolina, Columbia, SC 29208. Phone: (803) 777-7036. Fax: (803) 777-4002. E-mail: lovell{at}biol.sc.edu.

dagger Present address: Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831.

Applied and Environmental Microbiology, November 2001, p. 5308-5314, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5308-5314.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Dang, H., Luan, X., Zhao, J., Li, J. (2009). Diverse and Novel nifH and nifH-Like Gene Sequences in the Deep-Sea Methane Seep Sediments of the Okhotsk Sea. Appl. Environ. Microbiol. 75: 2238-2245 [Abstract] [Full Text]  
  • Welsh, A., Burke, D. J., Hahn, D. (2007). Analysis of Nitrogen-Fixing Members of the {varepsilon} Subclass of Proteobacteria in Salt Marsh Sediments. Appl. Environ. Microbiol. 73: 7747-7752 [Abstract] [Full Text]  
  • Flores-Mireles, A. L., Winans, S. C., Holguin, G. (2007). Molecular Characterization of Diazotrophic and Denitrifying Bacteria Associated with Mangrove Roots. Appl. Environ. Microbiol. 73: 7308-7321 [Abstract] [Full Text]  
  • Lalucat, J., Bennasar, A., Bosch, R., Garcia-Valdes, E., Palleroni, N. J. (2006). Biology of Pseudomonas stutzeri. Microbiol. Mol. Biol. Rev. 70: 510-547 [Abstract] [Full Text]  
  • Xie, C.-H., Yokota, A. (2006). Sphingomonas azotifigens sp. nov., a nitrogen-fixing bacterium isolated from the roots of Oryza sativa.. Int. J. Syst. Evol. Microbiol. 56: 889-893 [Abstract] [Full Text]  
  • Langlois, R. J., LaRoche, J., Raab, P. A. (2005). Diazotrophic Diversity and Distribution in the Tropical and Subtropical Atlantic Ocean. Appl. Environ. Microbiol. 71: 7910-7919 [Abstract] [Full Text]  
  • Dedysh, S. N., Ricke, P., Liesack, W. (2004). NifH and NifD phylogenies: an evolutionary basis for understanding nitrogen fixation capabilities of methanotrophic bacteria. Microbiology 150: 1301-1313 [Abstract] [Full Text]  
  • Jenkins, B. D., Steward, G. F., Short, S. M., Ward, B. B., Zehr, J. P. (2004). Fingerprinting Diazotroph Communities in the Chesapeake Bay by Using a DNA Macroarray. Appl. Environ. Microbiol. 70: 1767-1776 [Abstract] [Full Text]  
  • Yeager, C. M., Kornosky, J. L., Housman, D. C., Grote, E. E., Belnap, J., Kuske, C. R. (2004). Diazotrophic Community Structure and Function in Two Successional Stages of Biological Soil Crusts from the Colorado Plateau and Chihuahuan Desert. Appl. Environ. Microbiol. 70: 973-983 [Abstract] [Full Text]  
  • Janse, I., Meima, M., Kardinaal, W. E. A., Zwart, G. (2003). High-Resolution Differentiation of Cyanobacteria by Using rRNA-Internal Transcribed Spacer Denaturing Gradient Gel Electrophoresis. Appl. Environ. Microbiol. 69: 6634-6643 [Abstract] [Full Text]  
  • Mehta, M. P., Butterfield, D. A., Baross, J. A. (2003). Phylogenetic Diversity of Nitrogenase (nifH) Genes in Deep-Sea and Hydrothermal Vent Environments of the Juan de Fuca Ridge. Appl. Environ. Microbiol. 69: 960-970 [Abstract] [Full Text]  
  • Rosch, C., Mergel, A., Bothe, H. (2002). Biodiversity of Denitrifying and Dinitrogen-Fixing Bacteria in an Acid Forest Soil. Appl. Environ. Microbiol. 68: 3818-3829 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»