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Applied and Environmental Microbiology, November 2001, p. 5321-5324, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5321-5324.2001

Rapid Extraction of DNA From Escherichia coli and Cryptosporidium parvum for Use in PCR

James A. Higgins,* Mark C. Jenkins, Daniel R. Shelton, Ron Fayer, and Jeffrey S. Karns

U.S. Department of Agriculture-Agricultural Research Service, Beltsville, Maryland 20705

Received 17 July 2001/Accepted 23 August 2001

The Xtra Amp tube, Isocode paper, Instagene matrix, and PrepMan matrix methods were evaluated for their ability to rapidly extract PCR-quality DNAs from Escherichia coli O157:H7 and Cryptosporidium parvum. All methods provided satisfactory DNA from E. coli, and the Xtra Amp and Instagene reagents provided satisfactory DNA from C. parvum.

* Corresponding author. Mailing address: USDA-ARS, Rm. 202, Bldg. 173, 10300 Baltimore Blvd., Beltsville, MD 20705. Phone: (301) 504-6443. Fax: (301) 504-6608. E-mail: jhiggins{at}anri.barc.usda.gov.

Applied and Environmental Microbiology, November 2001, p. 5321-5324, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5321-5324.2001


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  • Margraf, R. L., Page, S., Erali, M., Wittwer, C. T. (2004). Single-Tube Method for Nucleic Acid Extraction, Amplification, Purification, and Sequencing. Clin. Chem. 50: 1755-1761 [Abstract] [Full Text]  


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