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Applied and Environmental Microbiology, November 2001, p. 5339-5342, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5339-5342.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Pyrosequencing as a Method for Grouping of Listeria monocytogenes Strains on the Basis of Single-Nucleotide Polymorphisms in the inlB Gene

Helle Unnerstad,1,* Henrik Ericsson,1 Anders Alderborn,2 Wilhelm Tham,1 Marie-Louise Danielsson-Tham,1 and Jens G. Mattsson3

Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, SE-750 07 Uppsala,1 Pyrosequencing AB, SE-752 28 Uppsala,2 and Department of Parasitology (SWEPAR), National Veterinary Institute, SE-751 89 Uppsala,3 Sweden

Received 5 March 2001/Accepted 6 August 2001

By using pyrosequencing (i.e., sequencing by synthesis) 106 strains of different serovars of Listeria monocytogenes were rapidly grouped into four categories based on nucleotide variations at positions 1575 and 1578 of the inlB gene. Strains of serovars 1/2a and 1/2c constituted one group, and strains of serovars 1/2b and 3b constituted another group, whereas serovar 4b strains were separated into two groups.

* Corresponding author. Mailing address: Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, P. O. Box 7009, SE-750 07 Uppsala, Sweden. Phone: 46 (0) 18 674261. Fax: 46 (0) 18 673334. E-mail: Helle.Unnerstad{at}sva.se.

Applied and Environmental Microbiology, November 2001, p. 5339-5342, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5339-5342.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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Copyright © 2001 by the American Society for Microbiology. All rights reserved.