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Applied and Environmental Microbiology, November 2001, p. 5343-5348, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5343-5348.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Diversity and Detection of Nitrate Assimilation Genes in Marine Bacteria

Andrew E. Allen,1,2 Melissa G. Booth,2 Marc E. Frischer,2,* Peter G. Verity,2 Jonathan P. Zehr,3 and Sabino Zani4

Institute of Ecology, University of Georgia, Athens, Georgia 306021; Skidaway Institute of Oceanography, Savannah, Georgia 314112; Ocean Sciences Department, University of California, Santa Cruz, Santa Cruz, California 950643; and Department of Biology, Rensselaer Polytechnic Institute, Troy, New York 12180-35904

Received 9 March 2001/Accepted 31 July 2001

A PCR approach was used to construct a database of nasA genes (called narB genes in cyanobacteria) and to detect the genetic potential for heterotrophic bacterial nitrate utilization in marine environments. A nasA-specific PCR primer set that could be used to selectively amplify the nasA gene from heterotrophic bacteria was designed. Using seawater DNA extracts obtained from microbial communities in the South Atlantic Bight, the Barents Sea, and the North Pacific Gyre, we PCR amplified and sequenced nasA genes. Our results indicate that several groups of heterotrophic bacterial nasA genes are common and widely distributed in oceanic environments.

* Corresponding author. Mailing address: Skidaway Institute of Oceanography, 10 Ocean Science Circle, Savannah, GA 31411. Phone: (912) 598-2441. Fax: (912) 598-2310. E-mail: frischer{at}skio.peachnet.edu.

Applied and Environmental Microbiology, November 2001, p. 5343-5348, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5343-5348.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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  • Langlois, R. J., LaRoche, J., Raab, P. A. (2005). Diazotrophic Diversity and Distribution in the Tropical and Subtropical Atlantic Ocean. Appl. Environ. Microbiol. 71: 7910-7919 [Abstract] [Full Text]  
  • Zehr, J. P., Ward, B. B. (2002). Nitrogen Cycling in the Ocean: New Perspectives on Processes and Paradigms. Appl. Environ. Microbiol. 68: 1015-1024 [Full Text]  


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