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Applied and Environmental Microbiology, December 2001, p. 5626-5633, Vol. 67, No. 12
Department of Microbiology, Technical
University of Denmark, DK-2800 Lyngby, Denmark
Received 6 August 2001/Accepted 1 October 2001
A promoter active in the late phase of the lytic cycle of
lactococcal bacteriophage TP901-1 has been identified. The promoter is
tightly regulated and requires the product of the phage TP901-1 orf29 for activity. A deletion analysis of the late
promoter region showed that a fragment as small as 99 bp contains both
the promoter and the region necessary for activation by ORF29. The
transcriptional start site of the promoter was identified by primer
extension to position 13073 on the TP901-1 genome, thus located 87 bp
downstream of orf29 in a 580-bp intergenic region
between orf29 and orf30. Furthermore, the
region located
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.12.5626-5633.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
An Activator of Transcription Regulates Phage
TP901-1 Late Gene Expression
85 to 61 bp upstream of the start site was shown to
be necessary for promoter activity. During infection, the transcript
arising from the late promoter is fully induced at 40 min
postinfection, and our results suggest that a certain level of ORF29
must be reached in order to activate transcription of the promoter.
Several lactococcal bacteriophages encode ORF29 homologous proteins,
indicating that late transcription may be controlled by a similar
mechanism in these phages. With the identification of this novel
regulator, our results suggest that within the P335 group of
lactococcal phages at least two regulatory systems controlling
transcription in the late stage of infection exist.
*
Corresponding author. Present address: Department of
Dairy and Food Science, Royal Veterinary and Agricultural University of
Denmark, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark. Phone: 45 35 28 32 71. Fax: 45 35 28 32 14. E-mail: lobr{at}kvl.dk.
Applied and Environmental Microbiology, December 2001, p. 5626-5633, Vol. 67, No. 12
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.12.5626-5633.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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