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Applied and Environmental Microbiology, December 2001, p. 5634-5642, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5634-5642.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of Enterocoliticin, a Phage Tail-Like Bacteriocin, and Its Effect on Pathogenic Yersinia enterocolitica Strains

Eckhard Strauch,1,* Heike Kaspar,1 Christoph Schaudinn,1 Petra Dersch,2 Kazimierz Madela,1 Christina Gewinner,1,dagger Stefan Hertwig,1 Jörg Wecke,1 and Bernd Appel1

Robert Koch Institut, 13353 Berlin,1 and Institut für Mikrobiologie, Freie Universität Berlin, 14195 Berlin,2 Germany

Received 15 May 2001/Accepted 19 September 2001

Yersinia enterocolitica 29930 (biogroup 1A; serogroup O:7,8) produces a bacteriocin, designated enterocoliticin, that shows inhibitory activity against enteropathogenic strains of Y. enterocolitica belonging to serogroups O:3, O:5,27 and O:9. Enterocoliticin was purified, and electron micrographs of enterocoliticin preparations revealed the presence of phage tail-like particles. The particles did not contain nucleic acids and showed contraction upon contact with susceptible bacteria. Enterocoliticin addition to logarithmic-phase cultures of susceptible bacterial strains led to a rapid dose-dependent reduction in CFU. Calorimetric measurements of the heat output of cultures of sensitive bacteria showed a complete loss of cellular metabolic activity immediately upon addition of enterocoliticin. Furthermore, a dose-dependent efflux of K+ ions into the medium was determined, indicating that enterocoliticin has channel-forming activity.

* Corresponding author. Mailing address: Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany. Phone: 49-1888-7542114. Fax: 49-1888-7542110. E-mail: strauche{at}rki.de.

dagger Present address: Institute for Biomedical Research, 60596 Frankfurt a.M., Germany.

Applied and Environmental Microbiology, December 2001, p. 5634-5642, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5634-5642.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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