AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Strauch, E.
Right arrow Articles by Appel, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Strauch, E.
Right arrow Articles by Appel, B.
Agricola
Right arrow Articles by Strauch, E.
Right arrow Articles by Appel, B.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2001, p. 5634-5642, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5634-5642.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of Enterocoliticin, a Phage Tail-Like Bacteriocin, and Its Effect on Pathogenic Yersinia enterocolitica Strains

Eckhard Strauch,1,* Heike Kaspar,1 Christoph Schaudinn,1 Petra Dersch,2 Kazimierz Madela,1 Christina Gewinner,1,dagger Stefan Hertwig,1 Jörg Wecke,1 and Bernd Appel1

Robert Koch Institut, 13353 Berlin,1 and Institut für Mikrobiologie, Freie Universität Berlin, 14195 Berlin,2 Germany

Received 15 May 2001/Accepted 19 September 2001

Yersinia enterocolitica 29930 (biogroup 1A; serogroup O:7,8) produces a bacteriocin, designated enterocoliticin, that shows inhibitory activity against enteropathogenic strains of Y. enterocolitica belonging to serogroups O:3, O:5,27 and O:9. Enterocoliticin was purified, and electron micrographs of enterocoliticin preparations revealed the presence of phage tail-like particles. The particles did not contain nucleic acids and showed contraction upon contact with susceptible bacteria. Enterocoliticin addition to logarithmic-phase cultures of susceptible bacterial strains led to a rapid dose-dependent reduction in CFU. Calorimetric measurements of the heat output of cultures of sensitive bacteria showed a complete loss of cellular metabolic activity immediately upon addition of enterocoliticin. Furthermore, a dose-dependent efflux of K+ ions into the medium was determined, indicating that enterocoliticin has channel-forming activity.


* Corresponding author. Mailing address: Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany. Phone: 49-1888-7542114. Fax: 49-1888-7542110. E-mail: strauche{at}rki.de.

dagger Present address: Institute for Biomedical Research, 60596 Frankfurt a.M., Germany.


Applied and Environmental Microbiology, December 2001, p. 5634-5642, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5634-5642.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2001 by the American Society for Microbiology. All rights reserved.