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Applied and Environmental Microbiology, December 2001, p. 5761-5770, Vol. 67, No. 12
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.12.5761-5770.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Visualization of N-Acylhomoserine
Lactone-Mediated Cell-Cell Communication between Bacteria Colonizing
the Tomato Rhizosphere
Anette
Steidle,1
Katja
Sigl,1
Regina
Schuhegger,2
Alexandra
Ihring,1
Markus
Schmid,1
Stephan
Gantner,3
Marion
Stoffels,3
Kathrin
Riedel,1
Michael
Givskov,4
Anton
Hartmann,3
Christian
Langebartels,2 and
Leo
Eberl1,*
Lehrstuhl für Mikrobiologie, Technische
Universität München, D-85350
Freising,1 and Institut für
Biochemische Pflanzenpathologie2 and
Institut für Bodenökologie,3
GSF-Forschungszentrum, D-85764 Neuherberg, Germany, and
Department of Microbiology, DTU, DK-2800 Lyngby,
Denmark4
Received 4 June 2001/Accepted 12 September 2001
Given that a large proportion of the bacteria colonizing the roots
of plants is capable of producing
N-acyl-L-homoserine lactone (AHL) molecules,
it appears likely that these bacterial pheromones may serve as signals
for communication between cells of different species. In this study, we
have developed and characterized novel Gfp-based monitor strains that
allow in situ visualization of AHL-mediated communication between
individual cells in the plant rhizosphere. For this purpose, three
Gfp-based AHL sensor plasmids that respond to different spectra of AHL
molecules were transferred into AHL-negative derivatives of
Pseudomonas putida IsoF and
Serratia liquefaciens MG1, two strains
that are capable of colonizing tomato roots. These AHL monitor strains
were used to visualize communication between defined bacterial
populations in the rhizosphere of axenically grown tomato plants.
Furthermore, we integrated into the chromosome of AHL-negative
P. putida strain F117 an AHL sensor
cassette that responds to the presence of long-chain AHLs with the
expression of Gfp. This monitor strain was used to demonstrate that the
indigenous bacterial community colonizing the roots of tomato plants
growing in nonsterile soil produces AHL molecules. The results strongly support the view that AHL signal molecules serve as a universal language for communication between the different bacterial populations of the rhizosphere consortium.
*
Corresponding author. Mailing address: Department of
Microbiology, Technical University of Munich, Am Hochanger 4, D-85350 Freising, Germany. Phone: 49 8161 715446. Fax: 49 8161 715475. E-mail:
EBERL{at}mikro.biologie.tu-muenchen.de.
Applied and Environmental Microbiology, December 2001, p. 5761-5770, Vol. 67, No. 12
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.12.5761-5770.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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