This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Steidle, A.
Right arrow Articles by Eberl, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Steidle, A.
Right arrow Articles by Eberl, L.
Agricola
Right arrow Articles by Steidle, A.
Right arrow Articles by Eberl, L.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2001, p. 5761-5770, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5761-5770.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Visualization of N-Acylhomoserine Lactone-Mediated Cell-Cell Communication between Bacteria Colonizing the Tomato Rhizosphere

Anette Steidle,1 Katja Sigl,1 Regina Schuhegger,2 Alexandra Ihring,1 Markus Schmid,1 Stephan Gantner,3 Marion Stoffels,3 Kathrin Riedel,1 Michael Givskov,4 Anton Hartmann,3 Christian Langebartels,2 and Leo Eberl1,*

Lehrstuhl für Mikrobiologie, Technische Universität München, D-85350 Freising,1 and Institut für Biochemische Pflanzenpathologie2 and Institut für Bodenökologie,3 GSF-Forschungszentrum, D-85764 Neuherberg, Germany, and Department of Microbiology, DTU, DK-2800 Lyngby, Denmark4

Received 4 June 2001/Accepted 12 September 2001

Given that a large proportion of the bacteria colonizing the roots of plants is capable of producing N-acyl-L-homoserine lactone (AHL) molecules, it appears likely that these bacterial pheromones may serve as signals for communication between cells of different species. In this study, we have developed and characterized novel Gfp-based monitor strains that allow in situ visualization of AHL-mediated communication between individual cells in the plant rhizosphere. For this purpose, three Gfp-based AHL sensor plasmids that respond to different spectra of AHL molecules were transferred into AHL-negative derivatives of Pseudomonas putida IsoF and Serratia liquefaciens MG1, two strains that are capable of colonizing tomato roots. These AHL monitor strains were used to visualize communication between defined bacterial populations in the rhizosphere of axenically grown tomato plants. Furthermore, we integrated into the chromosome of AHL-negative P. putida strain F117 an AHL sensor cassette that responds to the presence of long-chain AHLs with the expression of Gfp. This monitor strain was used to demonstrate that the indigenous bacterial community colonizing the roots of tomato plants growing in nonsterile soil produces AHL molecules. The results strongly support the view that AHL signal molecules serve as a universal language for communication between the different bacterial populations of the rhizosphere consortium.

* Corresponding author. Mailing address: Department of Microbiology, Technical University of Munich, Am Hochanger 4, D-85350 Freising, Germany. Phone: 49 8161 715446. Fax: 49 8161 715475. E-mail: EBERL{at}mikro.biologie.tu-muenchen.de.

Applied and Environmental Microbiology, December 2001, p. 5761-5770, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5761-5770.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Steindler, L., Bertani, I., De Sordi, L., Schwager, S., Eberl, L., Venturi, V. (2009). LasI/R and RhlI/R Quorum Sensing in a Strain of Pseudomonas aeruginosa Beneficial to Plants. Appl. Environ. Microbiol. 75: 5131-5140 [Abstract] [Full Text]  
  • de Werra, P., Baehler, E., Huser, A., Keel, C., Maurhofer, M. (2008). Detection of Plant-Modulated Alterations in Antifungal Gene Expression in Pseudomonas fluorescens CHA0 on Roots by Flow Cytometry. Appl. Environ. Microbiol. 74: 1339-1349 [Abstract] [Full Text]  
  • Dulla, G., Lindow, S. E. (2008). Quorum size of Pseudomonas syringae is small and dictated by water availability on the leaf surface. Proc. Natl. Acad. Sci. USA 105: 3082-3087 [Abstract] [Full Text]  
  • Delaspre, F., Nieto Penalver, C. G., Saurel, O., Kiefer, P., Gras, E., Milon, A., Boucher, C., Genin, S., Vorholt, J. A. (2007). The Ralstonia solanacearum pathogenicity regulator HrpB induces 3-hydroxy-oxindole synthesis. Proc. Natl. Acad. Sci. USA 104: 15870-15875 [Abstract] [Full Text]  
  • Vandamme, P., Opelt, K., Knochel, N., Berg, C., Schonmann, S., De Brandt, E., Eberl, L., Falsen, E., Berg, G. (2007). Burkholderia bryophila sp. nov. and Burkholderia megapolitana sp. nov., moss-associated species with antifungal and plant-growth-promoting properties. Int. J. Syst. Evol. Microbiol. 57: 2228-2235 [Abstract] [Full Text]  
  • Sanchez-Contreras, M., Bauer, W. D, Gao, M., Robinson, J. B, Allan Downie, J (2007). Quorum-sensing regulation in rhizobia and its role in symbiotic interactions with legumes. Phil Trans R Soc B 362: 1149-1163 [Abstract] [Full Text]  
  • Manefield, M., Whiteley, A. S (2007). Acylated homoserine lactones in the environment: chameleons of bioactivity. Phil Trans R Soc B 362: 1235-1240 [Abstract] [Full Text]  
  • Krick, A., Kehraus, S., Eberl, L., Riedel, K., Anke, H., Kaesler, I., Graeber, I., Szewzyk, U., Konig, G. M. (2007). A Marine Mesorhizobium sp. Produces Structurally Novel Long-Chain N-Acyl-L-Homoserine Lactones. Appl. Environ. Microbiol. 73: 3587-3594 [Abstract] [Full Text]  
  • DeAngelis, K. M., Firestone, M. K., Lindow, S. E. (2007). Sensitive Whole-Cell Biosensor Suitable for Detecting a Variety of N-Acyl Homoserine Lactones in Intact Rhizosphere Microbial Communities. Appl. Environ. Microbiol. 73: 3724-3727 [Abstract] [Full Text]  
  • Labbate, M., Zhu, H., Thung, L., Bandara, R., Larsen, M. R., Willcox, M. D. P., Givskov, M., Rice, S. A., Kjelleberg, S. (2007). Quorum-Sensing Regulation of Adhesion in Serratia marcescens MG1 Is Surface Dependent. J. Bacteriol. 189: 2702-2711 [Abstract] [Full Text]  
  • Penalver, C. G. N., Cantet, F., Morin, D., Haras, D., Vorholt, J. A. (2006). A Plasmid-Borne Truncated luxI Homolog Controls Quorum-Sensing Systems and Extracellular Carbohydrate Production in Methylobacterium extorquens AM1.. J. Bacteriol. 188: 7321-7324 [Abstract] [Full Text]  
  • WATT, M., SILK, W. K., PASSIOURA, J. B. (2006). Rates of Root and Organism Growth, Soil Conditions, and Temporal and Spatial Development of the Rhizosphere. ANN BOT (LOND) 97: 839-855 [Abstract] [Full Text]  
  • Wopperer, J., Cardona, S. T., Huber, B., Jacobi, C. A., Valvano, M. A., Eberl, L. (2006). A Quorum-Quenching Approach To Investigate the Conservation of Quorum-Sensing-Regulated Functions within the Burkholderia cepacia Complex. Appl. Environ. Microbiol. 72: 1579-1587 [Abstract] [Full Text]  
  • Riedel, K., Kothe, M., Kramer, B., Saeb, W., Gotschlich, A., Ammendola, A., Eberl, L. (2006). Computer-Aided Design of Agents That Inhibit the cep Quorum-Sensing System of Burkholderia cenocepacia. Antimicrob. Agents Chemother. 50: 318-323 [Abstract] [Full Text]  
  • Gao, M., Chen, H., Eberhard, A., Gronquist, M. R., Robinson, J. B., Rolfe, B. G., Bauer, W. D. (2005). sinI- and expR-Dependent Quorum Sensing in Sinorhizobium meliloti. J. Bacteriol. 187: 7931-7944 [Abstract] [Full Text]  
  • Williamson, L. L., Borlee, B. R., Schloss, P. D., Guan, C., Allen, H. K., Handelsman, J. (2005). Intracellular Screen To Identify Metagenomic Clones That Induce or Inhibit a Quorum-Sensing Biosensor. Appl. Environ. Microbiol. 71: 6335-6344 [Abstract] [Full Text]  
  • Huber, B., Feldmann, F., Kothe, M., Vandamme, P., Wopperer, J., Riedel, K., Eberl, L. (2004). Identification of a Novel Virulence Factor in Burkholderia cenocepacia H111 Required for Efficient Slow Killing of Caenorhabditis elegans. Infect. Immun. 72: 7220-7230 [Abstract] [Full Text]  
  • Espinosa-Urgel, M., Ramos, J.-L. (2004). Cell Density-Dependent Gene Contributes to Efficient Seed Colonization by Pseudomonas putida KT2440. Appl. Environ. Microbiol. 70: 5190-5198 [Abstract] [Full Text]  
  • Juhas, M., Wiehlmann, L., Huber, B., Jordan, D., Lauber, J., Salunkhe, P., Limpert, A. S., von Gotz, F., Steinmetz, I., Eberl, L., Tummler, B. (2004). Global regulation of quorum sensing and virulence by VqsR in Pseudomonas aeruginosa. Microbiology 150: 831-841 [Abstract] [Full Text]  
  • Teplitski, M., Chen, H., Rajamani, S., Gao, M., Merighi, M., Sayre, R. T., Robinson, J. B., Rolfe, B. G., Bauer, W. D. (2004). Chlamydomonas reinhardtii Secretes Compounds That Mimic Bacterial Signals and Interfere with Quorum Sensing Regulation in Bacteria. Plant Physiol. 134: 137-146 [Abstract] [Full Text]  
  • Gonzalez, J. E., Marketon, M. M. (2003). Quorum Sensing in Nitrogen-Fixing Rhizobia. Microbiol. Mol. Biol. Rev. 67: 574-592 [Abstract] [Full Text]  
  • Zhu, J., Chai, Y., Zhong, Z., Li, S., Winans, S. C. (2003). Agrobacterium Bioassay Strain for Ultrasensitive Detection of N-Acylhomoserine Lactone-Type Quorum-Sensing Molecules: Detection of Autoinducers in Mesorhizobium huakuii. Appl. Environ. Microbiol. 69: 6949-6953 [Abstract] [Full Text]  
  • Riedel, K., Talker-Huiber, D., Givskov, M., Schwab, H., Eberl, L. (2003). Identification and Characterization of a GDSL Esterase Gene Located Proximal to the swr Quorum-Sensing System of Serratia liquefaciens MG1. Appl. Environ. Microbiol. 69: 3901-3910 [Abstract] [Full Text]  
  • Peters, L., Konig, G. M., Wright, A. D., Pukall, R., Stackebrandt, E., Eberl, L., Riedel, K. (2003). Secondary Metabolites of Flustra foliacea and Their Influence on Bacteria. Appl. Environ. Microbiol. 69: 3469-3475 [Abstract] [Full Text]  
  • Aguilar, C., Bertani, I., Venturi, V. (2003). Quorum-Sensing System and Stationary-Phase Sigma Factor (rpoS) of the Onion Pathogen Burkholderia cepacia Genomovar I Type Strain, ATCC 25416. Appl. Environ. Microbiol. 69: 1739-1747 [Abstract] [Full Text]  
  • Steidle, A., Allesen-Holm, M., Riedel, K., Berg, G., Givskov, M., Molin, S., Eberl, L. (2002). Identification and Characterization of an N-Acylhomoserine Lactone-Dependent Quorum-Sensing System in Pseudomonas putida Strain IsoF. Appl. Environ. Microbiol. 68: 6371-6382 [Abstract] [Full Text]  
  • Berg, G., Roskot, N., Steidle, A., Eberl, L., Zock, A., Smalla, K. (2002). Plant-Dependent Genotypic and Phenotypic Diversity of Antagonistic Rhizobacteria Isolated from Different Verticillium Host Plants. Appl. Environ. Microbiol. 68: 3328-3338 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»