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Applied and Environmental Microbiology, December 2001, p. 5771-5779, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5771-5779.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Sequence Analysis of a 101-Kilobase Plasmid Required for Agar Degradation by a Microscilla Isolate

Zhenping Zhong,1 Aresa Toukdarian,1,* Donald Helinski,1 Vic Knauf,2,§ Sean Sykes,3 Jane E. Wilkinson,3 Colleen O'Bryne,3 Terry Shea,3 Craig DeLoughery,3,dagger and Ron Caspi1,Dagger

Division of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093-03221; Calgene, Inc., Davis, California 956162; and Cereon Genomics, Cambridge, Massachusetts 021393

Received 23 May 2001/Accepted 4 September 2001

An agar-degrading marine bacterium identified as a Microscilla species was isolated from coastal California marine sediment. This organism harbored a single 101-kb circular DNA plasmid designated pSD15. The complete nucleotide sequence of pSD15 was obtained, and sequence analysis indicated a number of genes putatively encoding a variety of enzymes involved in polysaccharide utilization. The most striking feature was the occurrence of five putative agarase genes. Loss of the plasmid, which occurred at a surprisingly high frequency, was associated with loss of agarase activity, supporting the sequence analysis results.

* Corresponding author. Mailing address: Division of Biology and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093-0322. Phone: (858) 534-2460. Fax: (858) 534-0559. E-mail: atoukdar{at}ucsd.edu.

dagger Present address: Aventis, Cambridge, MA 02139.

Dagger Present address: Pangene Corporation, Fremont, CA 94538.

§ Present address: Tilligen, Inc., Seattle, WA 98101.

Applied and Environmental Microbiology, December 2001, p. 5771-5779, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5771-5779.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.