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Applied and Environmental Microbiology, December 2001, p. 5819-5823, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5819-5823.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Improved Method for Detection of Vibrio parahaemolyticus in Seafood

Yukiko Hara-Kudo,1,* Tokuhiro Nishina,2 Hiroshi Nakagawa,3 Hirotaka Konuma,4 Junko Hasegawa,2 and Susumu Kumagai5

Department of Biomedical Food Research, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640,1 Department of Food and Nutrition, Tokai University Junior College, Shizuoka 420-8511,2 Tokyo Kenbikyoin Foundation, Chuo-ku, Tokyo 103-0015,3 Division of Microbiology, National Institute of Health Sciences, Setagaya-ku, Tokyo 158-8501,4 and The University of Tokyo, Graduate School of Agricultural and Life Sciences, Bunkyo-ku, Tokyo 113-8657,5 Japan

Received 24 May 2001/Accepted 25 September 2001

We have developed a new, effective procedure for detecting Vibrio parahaemolyticus in seafoods using enrichment and plating onto a chromogenic agar medium. Samples were cultured in salt Trypticase soy broth, which is a nonselective medium, and then a portion of the culture was cultured with salt polymyxin broth, which is a selective medium for V. parahaemolyticus. This two-step enrichment was more effective than the one-step enrichment in salt polymyxin broth alone. The enrichment cultures were then plated onto a new chromogenic agar containing substrates for beta-galactosidase. The V. parahaemolyticus colonies developed a purple color on this growth medium that distinguished them from other related bacterial strains. V. parahaemolyticus was isolated more frequently from naturally contaminated seafood samples using the chromogenic agar than thiosulfate citrate bile salts sucrose agar medium, which is currently used for the isolation of V. parahaemolyticus. Our findings suggest that this new enrichment and isolation scheme is more sensitive and accurate for identifying V. parahaemolyticus in seafood samples than previously used methods.

* Corresponding author. Mailing address: Department of Biomedical Food Research, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan Phone: 81 3 5285 1111. Fax: 81 3 5285 1176. E-mail: ykudo{at}nih.go.jp.

Applied and Environmental Microbiology, December 2001, p. 5819-5823, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5819-5823.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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