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Applied and Environmental Microbiology, December 2001, p. 5844-5848, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5844-5848.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

New Method for Evaluation of Virucidal Activity of Antiseptics and Disinfectants

Georgios T. Papageorgiou,1 Laura Mocé-Llivina,2 and Juan Jofre2,*

Microbiology Section, State General Laboratory, Ministry of Health, Nicosia, Cyprus,1 and Department of Microbiology, University of Barcelona, Barcelona, Spain2

Received 29 May 2001/Accepted 26 September 2001

Counting culturable viruses adsorbed to cellulose nitrate filters (the VIRADEN method) is proposed as a simple procedure for the evaluation of the virucidal activity of antiseptics and disinfectants. The virucidal activities of two different doses of iodine, chlorine, glutaraldehyde, and chlorhexidine digluconate on poliovirus 1 were tested with a standardized procedure and with the VIRADEN method. The two procedures assayed provided similar results.

* Corresponding author. Mailing address: Avda. Diagonal, 645, 0828 Barcelona, Spain. Phone: 34 93 402 14 87. Fax: 34 93 411 05 92. E-mail: joan{at}porthos.bio.ub.es.

Applied and Environmental Microbiology, December 2001, p. 5844-5848, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5844-5848.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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