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Applied and Environmental Microbiology, December 2001, p. 5855-5858, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5855-5858.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Toxicity Analysis of N- and C-Terminus-Deleted Vegetative Insecticidal Protein from Bacillus thuringiensis

A. Selvapandiyan,1 N. Arora,1 R. Rajagopal,1 S. K. Jalali,2 T. Venkatesan,2 S. P. Singh,2 and Raj K. Bhatnagar1,*

International Centre for Genetic Engineering and Biotechnology, New Delhi 110 067,1 and Biotechnology Laboratory, Project Directorate of Biological Control, Bangalore 560 024,2 India

Received 23 March 2001/Accepted 3 September 2001

A vegetative insecticidal protein (VIP)-encoding gene from a local isolate of Bacillus thuringiensis has been cloned, sequenced, and expressed in Escherichia coli. The expressed protein shows insecticidal activity against several lepidopteran pests but is ineffective against Agrotis ipsilon. Comparison of the amino acid sequence with those of reported VIPs revealed a few differences. Analysis of insecticidal activity with N- and C-terminus deletion mutants suggests a differential mode of action of VIP against different pests.

* Corresponding author. Mailing address: International Centre for Genetic Engineering and Biotechnology, P.O. Box 10504, Aruna Asaf Ali Marg, New Delhi 110 067, India. Phone: 91-11-6181242. Fax: 91-11-6162316. E-mail: raj{at}icgeb.res.in or rajbhatnagar{at}hotmail.com.

Applied and Environmental Microbiology, December 2001, p. 5855-5858, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5855-5858.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.