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Applied and Environmental Microbiology, February 2001, p. 1001-1003, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.1001-1003.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Molecular Characterization of the Iron-Hydroxamate Uptake System in Staphylococcus aureus

Guillermo Cabrera,dagger Anming Xiong,Dagger Michelle Uebel, Vineet K. Singh, and Radheshyam K. Jayaswal*

Microbiology Group, Department of Biological Sciences, Illinois State University, Normal, Illinois 61790-4120

Received 23 October 2000/Accepted 19 November 2000

To investigate iron uptake, a chromosomal locus containing three consecutive open reading frames, designated fhuC, fhuB, and fhuD, was identified in Staphylococcus aureus. Whereas the fhuC gene encodes an ATP-binding protein, fhuB and fhuD code for ferrichrome permeases and thus resemble an ATP-binding cassette transporter. A fhuB knockout mutant showed impaired uptake of iron bound to the siderophores but not of ferric chloride, suggesting that this operon is specific for siderophore-mediated iron uptake.


* Corresponding author. Mailing address: Microbiology Group, Department of Biological Sciences, Illinois State University, Normal, IL 61790-4120. Phone: (309) 438-5128. Fax: (309) 438-3722. E-mail: drjay{at}ilstu.edu.

dagger Present address: CEACA, UAQ, Centro Universitario, Queretaro, Qro. 76010, Mexico.

Dagger Present address: Dept. of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305.


Applied and Environmental Microbiology, February 2001, p. 1001-1003, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.1001-1003.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



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Copyright © 2001 by the American Society for Microbiology. All rights reserved.