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Applied and Environmental Microbiology, February 2001, p. 1008-1010, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.1008-1010.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of Rhizobial Isolates of Phaseolus vulgaris by Staircase Electrophoresis of Low-Molecular-Weight RNA

Encarna Velázquez,1,* Esperanza Martínez-Romero,2 Dulce Nombre Rodríguez-Navarro,3 Martha E. Trujillo,1 Antonio Daza,3 Pedro F. Mateos,1 Eustoquio Martínez-Molina,1 and Peter van Berkum4

Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca,1 and Centro de Investigación y Desarollo Agrario. CIDA "Las Torres y Tomejil," Seville,3 Spain; Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México2; and U.S. Department of Agriculture, Agricultural Research Service, Soybean and Alfalfa Research Laboratory, Beltsville, Maryland 207054

Received 10 July 2000/Accepted 13 November 2000

Low-molecular-weight (LMW) RNA molecules were analyzed to characterize rhizobial isolates that nodulate the common bean growing in Spain. Since LMW RNA profiles, determined by staircase electrophoresis, varied across the rhizobial species nodulating beans, we demonstrated that bean isolates recovered from Spanish soils presumptively could be characterized as Rhizobium etli, Rhizobium gallicum, Rhizobium giardinii, Rhizobium leguminosarum bv. viciae and bv. trifolii, and Sinorhizobium fredii.

* Corresponding author. Mailing address: Departamento de Microbiologia y Genetica, Universidad de Salamanca, Edificio Departamental, Avda. Campo charro s/n, 37007 Salamanca, Spain. Phone: 34 923 294532. Fax: 34 923 224876. E-mail: evp{at}gugu.usal.es.

Applied and Environmental Microbiology, February 2001, p. 1008-1010, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.1008-1010.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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