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Applied and Environmental Microbiology, February 2001, p. 1011-1014, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.1011-1014.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Synthesis of a Low-Molecular-Weight Form of Exopolysaccharide by Bradyrhizobium japonicum USDA 110

Heather A. Louch1,dagger and Karen J. Miller1,2,*

Intercollege Graduate Program in Genetics1 and Department of Food Science,2 The Pennsylvania State University, University Park, Pennsylvania 16802

Received 3 July 2000/Accepted 4 December 2000

A novel extracellular low-molecular-weight polysaccharide was detected as a contaminant within extracellular cyclic beta -1,6-beta -1,3-glucan preparations from Bradyrhizobium japonicum USDA 110 cultures. Compositional analysis, methylation analysis, and nuclear magnetic resonance analysis revealed that this low-molecular-weight polysaccharide was composed of the same pentasaccharide repeating unit previously described for the high-molecular-weight form of the exopolysaccharide (EPS) synthesized by B. japonicum strains. Mass spectrometry analysis indicated that the size of this low-molecular-weight form of EPS was consistent with a dimeric form of the pentasaccharide repeating unit.

* Corresponding author. Mailing address: 105 Borland Laboratory, Department of Food Science, The Pennsylvania State University, University Park, PA 16802. Phone: (814) 863-2954. Fax: (814) 863-6132. E-mail: kjm3{at}psu.edu.

dagger Present address: Laboratory of Bacterial Toxins, Center for Biologics Evaluation and Research, Food and Drug Administration, 8800 Rockville Pike, Bethesda, MD 20892.

Applied and Environmental Microbiology, February 2001, p. 1011-1014, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.1011-1014.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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