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Applied and Environmental Microbiology, February 2001, p. 1015-1019, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.1015-1019.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Nickel-Resistance-Based Minitransposons: New Tools for Genetic Manipulation of Environmental Bacteria

Safieh Taghavi,1 Hilde Delanghe,1 Cindy Lodewyckx,1,2 Max Mergeay,1 and Daniël van der Lelie1,*

Vlaamse Instelling voor Technologisch Onderzoek (Vito), Environmental Technology Centre, Boeretang 200, 2400 Mol,1 and Limburgs Universitair Centrum, Environmental Biology, Universitaire Campus, B3590 Diepenbeek,2 Belgium

Received 5 October 2000/Accepted 27 November 2000

The ncc and nre nickel resistance determinants from Ralstonia eutropha-like strain 31A were used to construct mini-Tn5 transposons. Broad host expression of nickel resistance was observed for the nre minitransposons in members of the alpha , beta , and gamma  subclasses of the Proteobacteria, while the ncc minitransposons expressed nickel resistance only in R. eutropha-like strains.

* Corresponding author. Mailing address: Vlaamse Instelling voor Technologisch Onderzoek (Vito), Environmental Technology Centre, Boeretang 200, 2400 Mol, Belgium. Phone: 32-14-33.51.66. Fax: 32-14-58.05.23. E-mail: niels.vanderlelie{at}vito.be.

Applied and Environmental Microbiology, February 2001, p. 1015-1019, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.1015-1019.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.