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Applied and Environmental Microbiology, February 2001, p. 1015-1019, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.1015-1019.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Nickel-Resistance-Based Minitransposons: New Tools for Genetic Manipulation of Environmental Bacteria

Safieh Taghavi,1 Hilde Delanghe,1 Cindy Lodewyckx,1,2 Max Mergeay,1 and Daniël van der Lelie1,*

Vlaamse Instelling voor Technologisch Onderzoek (Vito), Environmental Technology Centre, Boeretang 200, 2400 Mol,1 and Limburgs Universitair Centrum, Environmental Biology, Universitaire Campus, B3590 Diepenbeek,2 Belgium

Received 5 October 2000/Accepted 27 November 2000

The ncc and nre nickel resistance determinants from Ralstonia eutropha-like strain 31A were used to construct mini-Tn5 transposons. Broad host expression of nickel resistance was observed for the nre minitransposons in members of the alpha , beta , and gamma  subclasses of the Proteobacteria, while the ncc minitransposons expressed nickel resistance only in R. eutropha-like strains.

* Corresponding author. Mailing address: Vlaamse Instelling voor Technologisch Onderzoek (Vito), Environmental Technology Centre, Boeretang 200, 2400 Mol, Belgium. Phone: 32-14-33.51.66. Fax: 32-14-58.05.23. E-mail: niels.vanderlelie{at}vito.be.

Applied and Environmental Microbiology, February 2001, p. 1015-1019, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.1015-1019.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Taghavi, S., Garafola, C., Monchy, S., Newman, L., Hoffman, A., Weyens, N., Barac, T., Vangronsveld, J., van der Lelie, D. (2009). Genome Survey and Characterization of Endophytic Bacteria Exhibiting a Beneficial Effect on Growth and Development of Poplar Trees. Appl. Environ. Microbiol. 75: 748-757 [Abstract] [Full Text]  
  • Chaintreuil, C., Rigault, F., Moulin, L., Jaffre, T., Fardoux, J., Giraud, E., Dreyfus, B., Bailly, X. (2007). Nickel Resistance Determinants in Bradyrhizobium Strains from Nodules of the Endemic New Caledonia Legume Serianthes calycina. Appl. Environ. Microbiol. 73: 8018-8022 [Abstract] [Full Text]  
  • Taghavi, S., Barac, T., Greenberg, B., Borremans, B., Vangronsveld, J., van der Lelie, D. (2005). Horizontal Gene Transfer to Endogenous Endophytic Bacteria from Poplar Improves Phytoremediation of Toluene. Appl. Environ. Microbiol. 71: 8500-8505 [Abstract] [Full Text]  


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