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Applied and Environmental Microbiology, February 2001, p. 586-590, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.586-590.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Viability of Giardia intestinalis Cysts and Viability and Sporulation State of Cyclospora cayetanensis Oocysts Determined by Electrorotation

C. Dalton,1 A. D. Goater,1,* R. Pethig,1 and H. V. Smith2

Institute of Molecular and Biomolecular Electronics, University of Wales, Bangor, Gwynedd LL57 1UT,1 and Scottish Parasite Diagnostic Laboratory, Stobhill Hospital, Glasgow G21 3UW,2 United Kingdom

Received 26 June 2000/Accepted 29 September 2000

Electrorotation is a noninvasive technique that is capable of detecting changes in the morphology and physicochemical properties of microorganisms. Electrorotation studies are reported for two intestinal parasites, Giardia intestinalis and Cyclospora cayetanensis. It is concluded that viable and nonviable G. intestinalis cysts can be differentiated by this technique, and support for this conclusion was obtained using a fluorogenic vital dye assay and morphological indicators. The viability of C. cayetanensis oocysts (for which no vital dye assay is currently available) can also be determined by electrorotation, as can their sporulation state. Modeling of the electrorotational response of these organisms was used to determine their dielectric properties and to gain an insight into the changes occurring within them. Electrorotation offers a new, simple, and rapid method for determining the viability of parasites in potable water and food products and as such has important healthcare implications.

* Corresponding author. Mailing address: Institute of Molecular and Biomolecular Electronics, University of Wales, Dean St., Bangor, Gwynedd LL57 1UT, United Kingdom. Phone: 44 (0) 1248 351151, ext. 2711. Fax: 44 (0) 1248 361429. E-mail: agoater{at}sees.bangor.ac.uk.

Applied and Environmental Microbiology, February 2001, p. 586-590, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.586-590.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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