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Applied and Environmental Microbiology, February 2001, p. 888-894, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.888-894.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Diversity of Sulfur Isotope Fractionations by Sulfate-Reducing Prokaryotes†

Jan Detmers,1,* Volker Brüchert,1,* Kirsten S. Habicht,2 and Jan Kuever1

Max-Planck-Institute for Marine Microbiology, 28359 Bremen, Germany,1 and Institute of Biology, University of Southern Denmark, Odense University, 5230 Odense M, Denmark2

Received 4 August 2000/Accepted 27 November 2000

Batch culture experiments were performed with 32 different sulfate-reducing prokaryotes to explore the diversity in sulfur isotope fractionation during dissimilatory sulfate reduction by pure cultures. The selected strains reflect the phylogenetic and physiologic diversity of presently known sulfate reducers and cover a broad range of natural marine and freshwater habitats. Experimental conditions were designed to achieve optimum growth conditions with respect to electron donors, salinity, temperature, and pH. Under these optimized conditions, experimental fractionation factors ranged from 2.0 to 42.0per thousand . Salinity, incubation temperature, pH, and phylogeny had no systematic effect on the sulfur isotope fractionation. There was no correlation between isotope fractionation and sulfate reduction rate. The type of dissimilatory bisulfite reductase also had no effect on fractionation. Sulfate reducers that oxidized the carbon source completely to CO2 showed greater fractionations than sulfate reducers that released acetate as the final product of carbon oxidation. Different metabolic pathways and variable regulation of sulfate transport across the cell membrane all potentially affect isotope fractionation. Previous models that explained fractionation only in terms of sulfate reduction rates appear to be oversimplified. The species-specific physiology of each sulfate reducer thus needs to be taken into account to understand the regulation of sulfur isotope fractionation during dissimilatory sulfate reduction.

* Corresponding author. Mailing address: Max-Planck-Institute for Marine Microbiology, Celsiusstr. 1, 28359 Bremen, Germany. Phone: 49-421-2028-734. Fax: 49-421-2028-690. E-mail: jdetmers{at}mpi-bremen.de and vbrucher{at}mpi-bremen.de.

dagger This paper is publication no. 139 of the Priority Program 546 "Geochemical processes with long-term effects in anthropogenically-affected seepage and groundwater" by the Deutsche Forschungsgemeinschaft.

Applied and Environmental Microbiology, February 2001, p. 888-894, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.888-894.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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