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Applied and Environmental Microbiology, March 2001, p. 1185-1189, Vol. 67, No. 3
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.3.1185-1189.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Evidence for a Genetically Stable Strain of Campylobacter jejuni

G. Manning,1,* B. Duim,2 T. Wassenaar,2 J. A. Wagenaar,2 A. Ridley,1 and D. G. Newell1

Veterinary Laboratories Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB, United Kingdom,1 and ID-Lelystad, Lelystad, The Netherlands2

Received 14 August 2000/Accepted 27 November 2000

The genetic stability of selected epidemiologically linked strains of Campylobacter jejuni during outbreak situations was investigated by using subtyping techniques. Strains isolated from geographically related chicken flock outbreaks in 1998 and from a human outbreak in 1981 were investigated. There was little similarity in the strains obtained from the different chicken flock outbreaks; however, the strains from each of three chicken outbreaks, including strains isolated from various environments, were identical as determined by fla typing, amplified fragment length polymorphism (AFLP) analysis, and pulsed-field gel electrophoresis, which confirmed the genetic stability of these strains during the short time courses of chicken flock outbreaks. The human outbreak samples were compared with strain 81116, which originated from the same outbreak but has since undergone innumerable laboratory passages. Two main AFLP profiles were recognized from this outbreak, which confirmed the serotyping results obtained at the time of the outbreak. The major type isolated from this outbreak (serotype P6:L6) was exemplified by strain 81116. Despite the long existence of strain 81116 as a laboratory strain, the AFLP profile of this strain was identical to the profiles of all the other historical P6:L6 strains from the outbreak, indicating that the genotype has remained stable for almost 20 years. Interestingly, the AFLP profiles of the P6:L6 group of strains from the human outbreak and the strains from one of the recent chicken outbreaks were also identical. This similarity suggests that some clones of C. jejuni remain genetically stable in completely different environments over long periods of time and considerable geographical distances.

* Corresponding author. Mailing address: Veterinary Laboratories Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. Phone: (44) 1932 357 738. Fax: (44) 1932 357 595. E-mail: gmanning.cvl.wood{at}gtnet.gov.uk.

Applied and Environmental Microbiology, March 2001, p. 1185-1189, Vol. 67, No. 3
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.3.1185-1189.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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