This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Randall, L. P.
Right arrow Articles by Woodward, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Randall, L. P.
Right arrow Articles by Woodward, M. J.
Agricola
Right arrow Articles by Randall, L. P.
Right arrow Articles by Woodward, M. J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2001, p. 1190-1197, Vol. 67, No. 3
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.3.1190-1197.2001

Multiple Antibiotic Resistance (mar) Locus in Salmonella enterica Serovar Typhimurium DT104

Luke P. Randall and Martin J. Woodward*

Department of Bacterial Disease, Veterinary Laboratories Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB, United Kingdom

Received 16 August 2000/Accepted 2 January 2001

In order to understand the role of the mar locus in Salmonella with regard to multiple antibiotic resistance, cyclohexane resistance, and outer membrane protein F (OmpF) regulation, a marA::gfp reporter mutant was constructed in an antibiotic-sensitive Salmonella enterica serovar Typhimurium DT104 background. Salicylate induced marA, whereas a number of antibiotics, disinfectants, and various growth conditions did not. Increased antibiotic resistance was observed upon salicylate induction, although this was shown to be by both mar-dependent and mar-independent pathways. Cyclohexane resistance, however, was induced by salicylate by a mar-dependent pathway. Complementation studies with a plasmid that constitutively expressed marA confirmed the involvement of mar in Salmonella with low-level antibiotic resistance and cyclohexane resistance, although the involvement of mar in down regulation of OmpF was unclear. However, marA overexpression did increase the expression of a ca. 50-kDa protein, but its identity remains to be elucidated. Passage of the marA::gfp reporter mutant with increasing levels of tetracycline, a method reported to select for mar mutants in Escherichia coli, led to both multiple-antibiotic and cyclohexane resistance. Collectively, these data indicate that low-level antibiotic resistance, cyclohexane resistance, and modulation of OMPs in Salmonella, as in E. coli, can occur in both a mar-dependent and mar-independent manner.

* Corresponding author. Mailing address: Department of Bacterial Disease, Veterinary Laboratories Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. Phone: 44 (0) 1932 357582. Fax: 44 (0) 1932 347046. E-mail: M.J.Wooward{at}vla.maff.gsi.gov.uk.

Applied and Environmental Microbiology, March 2001, p. 1190-1197, Vol. 67, No. 3
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.3.1190-1197.2001


This article has been cited by other articles:

  • O'Regan, E., Quinn, T., Pages, J.-M., McCusker, M., Piddock, L., Fanning, S. (2009). Multiple Regulatory Pathways Associated with High-Level Ciprofloxacin and Multidrug Resistance in Salmonella enterica Serovar Enteritidis: Involvement of ramA and Other Global Regulators. Antimicrob. Agents Chemother. 53: 1080-1087 [Abstract] [Full Text]  
  • Abouzeed, Y. M., Baucheron, S., Cloeckaert, A. (2008). ramR Mutations Involved in Efflux-Mediated Multidrug Resistance in Salmonella enterica Serovar Typhimurium. Antimicrob. Agents Chemother. 52: 2428-2434 [Abstract] [Full Text]  
  • Stromqvist Meuzelaar, L., Hopkins, K., Liebana, E., Brookes, A. J. (2007). DNA Diagnostics by Surface-Bound Melt-Curve Reactions. J. Mol. Diagn. 9: 30-41 [Abstract] [Full Text]  
  • Piddock, L. J. V. (2006). Clinically Relevant Chromosomally Encoded Multidrug Resistance Efflux Pumps in Bacteria. Clin. Microbiol. Rev. 19: 382-402 [Abstract] [Full Text]  
  • Ricci, V., Tzakas, P., Buckley, A., Coldham, N. C., Piddock, L. J. V. (2006). Ciprofloxacin-Resistant Salmonella enterica Serovar Typhimurium Strains Are Difficult To Select in the Absence of AcrB and TolC. Antimicrob. Agents Chemother. 50: 38-42 [Abstract] [Full Text]  
  • Burgos, J. M., Ellington, B. A., Varela, M. F. (2005). Presence of Multidrug-Resistant Enteric Bacteria in Dairy Farm Topsoil. J DAIRY SCI 88: 1391-1398 [Abstract] [Full Text]  
  • Randall, L. P., Cooles, S. W., Piddock, L. J. V., Woodward, M. J. (2004). Effect of triclosan or a phenolic farm disinfectant on the selection of antibiotic-resistant Salmonella enterica. J Antimicrob Chemother 54: 621-627 [Abstract] [Full Text]  
  • Prouty, A. M., Brodsky, I. E., Falkow, S., Gunn, J. S. (2004). Bile-salt-mediated induction of antimicrobial and bile resistance in Salmonella typhimurium. Microbiology 150: 775-783 [Abstract] [Full Text]  
  • Delsol, A. A., Woodward, M. J., Roe, J. M. (2004). Effect of a 5 day enrofloxacin treatment on Salmonella enterica serotype Typhimurium DT104 in the pig. J Antimicrob Chemother 53: 396-398 [Abstract] [Full Text]  
  • Chen, S., Zhao, S., White, D. G., Schroeder, C. M., Lu, R., Yang, H., McDermott, P. F., Ayers, S., Meng, J. (2004). Characterization of Multiple-Antimicrobial-Resistant Salmonella Serovars Isolated from Retail Meats. Appl. Environ. Microbiol. 70: 1-7 [Abstract] [Full Text]  
  • Hautefort, I., Proenca, M. J., Hinton, J. C. D. (2003). Single-Copy Green Fluorescent Protein Gene Fusions Allow Accurate Measurement of Salmonella Gene Expression In Vitro and during Infection of Mammalian Cells. Appl. Environ. Microbiol. 69: 7480-7491 [Abstract] [Full Text]  
  • Gayet, S., Chollet, R., Molle, G., Pages, J.-M., Chevalier, J. (2003). Modification of Outer Membrane Protein Profile and Evidence Suggesting an Active Drug Pump in Enterobacter aerogenes Clinical Strains. Antimicrob. Agents Chemother. 47: 1555-1559 [Abstract] [Full Text]  
  • Liebana, E., Clouting, C., Cassar, C. A., Randall, L. P., Walker, R. A., Threlfall, E. J., Clifton-Hadley, F. A., Ridley, A. M., Davies, R. H. (2002). Comparison of gyrA Mutations, Cyclohexane Resistance, and the Presence of Class I Integrons in Salmonella enterica from Farm Animals in England and Wales. J. Clin. Microbiol. 40: 1481-1486 [Abstract] [Full Text]  
  • RANDALL, L.P., WOODWARD, M.J. (2001). Role of the mar locus in virulence of Salmonella enterica serovar Typhimurium DT104 in chickens. J Med Microbiol 50: 770-779 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»