AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Elasri, M.
Right arrow Articles by Dessaux, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Elasri, M.
Right arrow Articles by Dessaux, Y.
Agricola
Right arrow Articles by Elasri, M.
Right arrow Articles by Dessaux, Y.

Applied and Environmental Microbiology, March 2001, p. 1198-1209, Vol. 67, No. 3
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.3.1198-1209.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Acyl-Homoserine Lactone Production Is More Common among Plant-Associated Pseudomonas spp. than among Soilborne Pseudomonas spp.†

Miena Elasri,1 Sandrine Delorme,2 Philippe Lemanceau,2 Gordon Stewart,3 Bridget Laue,3 Eric Glickmann,1 Phil M. Oger,1 and Yves Dessaux1,*

Institut des Sciences Végétales, CNRS UPR040, 91198 Gif-sur-Yvette Cedex,1 and UMR INRA-Université de Bourgogne BBCE-IPM, CMSE-INRA, 21034 Dijon Cedex,2 France, and University of Nottingham, Leicester, Sutton Bonington, United Kingdom3

Received 24 August 2000/Accepted 12 December 2000

A total of 137 soilborne and plant-associated bacterial strains belonging to different Pseudomonas species were tested for their ability to synthesize N-acyl-homoserine lactones (NAHL). Fifty-four strains synthesized NAHL. Interestingly, NAHL production appears to be more common among plant-associated than among soilborne Pseudomonas spp. Indeed, 40% of the analyzed Pseudomonas syringae strains produced NAHL which were identified most often as the short-chain NAHL, N-hexanoyl-L-homoserine lactone, N-(3-oxo-hexanoyl)-homoserine lactone, and N-(3-oxo-octanoyl)-L-homoserine lactone (no absolute correlation between genomospecies of P. syringae and their ability to produce NAHL could be found). Six strains of fluorescent pseudomonads, belonging to the species P. chlororaphis, P. fluorescens, and P. putida, isolated from the plant rhizosphere produced different types of NAHL. In contrast, none of the strains isolated from soil samples were shown to produce NAHL. The gene encoding the NAHL synthase in P. syringae pv. maculicola was isolated by complementation of an NAHL-deficient Chromobacterium mutant. Sequence analysis revealed the existence of a luxI homologue that we named psmI. This gene is sufficient to confer NAHL synthesis upon its bacterial host and has strong homology to psyI and ahlI, two genes involved in NAHL production in P. syringae pv. tabaci and P. syringae pv. syringae, respectively. We identified another open reading frame that we termed psmR, transcribed convergently in relation to psmI and partly overlapping psmI; this gene encodes a putative LuxR regulatory protein. This gene organization, with luxI and luxR homologues facing each other and overlapping, has been found so far only in the enteric bacteria Erwinia and Pantoea and in the related species P. syringae pv. tabaci.

* Corresponding author. Mailing address: ISV, Bâtiment 23, CNRS, Ave. de la Terrasse, 91198 Gif-sur-Yvette Cedex, France. Phone: 33 1 6982 3690. Fax: 33 1 6982 3695. E-mail: yves.dessaux{at}isv.cnrs-gir.fr.

dagger This paper is dedicated to the memory of Gordon Stewart.

Applied and Environmental Microbiology, March 2001, p. 1198-1209, Vol. 67, No. 3
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.3.1198-1209.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2001 by the American Society for Microbiology. All rights reserved.