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Applied and Environmental Microbiology, March 2001, p. 1284-1291, Vol. 67, No. 3
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.3.1284-1291.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Spiroplasma Symbiont of the Pea Aphid, Acyrthosiphon pisum (Insecta: Homoptera)

Takema Fukatsu,1,* Tsutomu Tsuchida,1,2 Naruo Nikoh,1,3 and Ryuichi Koga1

National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology, Tsukuba 305-8566,1 Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Tokyo 153-8902,2 and Bio-Oriented Technology Research Advancement Institution, Omiya 331-8537,3 Japan

Received 13 October 2000/Accepted 2 January 2001

From a laboratory strain of the pea aphid, Acyrthosiphon pisum, we discovered a previously unknown facultative endosymbiotic bacterium. Molecular phylogenetic analysis based on 16S ribosomal DNA revealed that the bacterium is a member of the genus Spiroplasma. The Spiroplasma organism showed stable vertical transmission through successive generations of the host. Injection of hemolymph from infected insects into uninfected insects established a stable infection in the recipients. The Spiroplasma symbiont exhibited negative effects on growth, reproduction, and longevity of the host, particularly in older adults. Of 58 clonal strains of A. pisum established from natural populations in central Japan, 4 strains possessed the Spiroplasma organism.

* Corresponding author. Mailing address: National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology, 1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. Phone: 81-298-61-6087. Fax: 81-298-61-6080. E-mail: fukatsu{at}nibh.go.jp.

Applied and Environmental Microbiology, March 2001, p. 1284-1291, Vol. 67, No. 3
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.3.1284-1291.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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