This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Leaphart, A. B.
Right arrow Articles by Lovell, C. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Leaphart, A. B.
Right arrow Articles by Lovell, C. R.
Agricola
Right arrow Articles by Leaphart, A. B.
Right arrow Articles by Lovell, C. R.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2001, p. 1392-1395, Vol. 67, No. 3
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.3.1392-1395.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Recovery and Analysis of Formyltetrahydrofolate Synthetase Gene Sequences from Natural Populations of Acetogenic Bacteria

Adam B. Leaphart and Charles R. Lovell*

Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208

Received 22 August 2000/Accepted 13 December 2000

Primers for PCR amplification of partial (1,102 of 1,680 bp) formyltetrahydrofolate synthetase (FTHFS) gene sequences were developed and tested. Partial FTHFS sequences were successfully amplified from DNA from pure cultures of known acetogens, from other FTHFS-producing organisms, from the roots of the smooth cordgrass, Spartina alterniflora, and from fresh horse manure. The amplimers recovered were cloned, their nucleotide sequences were determined, and their translated amino acid sequences were used to construct phylogenetic trees. We found that FTHFS sequences from homoacetogens formed a monophyletic cluster that did not contain sequences from nonhomoacetogens and that FTHFS sequences appear to be informative regarding major physiological features of FTHFS-producing organisms.

* Corresponding author. Mailing address: Department of Biological Sciences, University of South Carolina, Columbia, SC 29208. Phone: (803) 777-7036. Fax: (803) 777-4002. E-mail: lovell{at}biol.sc.edu.

Applied and Environmental Microbiology, March 2001, p. 1392-1395, Vol. 67, No. 3
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.3.1392-1395.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Vianna, M. E., Holtgraewe, S., Seyfarth, I., Conrads, G., Horz, H. P. (2008). Quantitative Analysis of Three Hydrogenotrophic Microbial Groups, Methanogenic Archaea, Sulfate-Reducing Bacteria, and Acetogenic Bacteria, within Plaque Biofilms Associated with Human Periodontal Disease. J. Bacteriol. 190: 3779-3785 [Abstract] [Full Text]  
  • Chauhan, A., Ogram, A. (2006). Fatty Acid-Oxidizing Consortia along a Nutrient Gradient in the Florida Everglades. Appl. Environ. Microbiol. 72: 2400-2406 [Abstract] [Full Text]  
  • Salmassi, T. M., Leadbetter, J. R. (2003). Analysis of genes of tetrahydrofolate-dependent metabolism from cultivated spirochaetes and the gut community of the termite Zootermopsis angusticollis. Microbiology 149: 2529-2537 [Abstract] [Full Text]  
  • Leaphart, A. B., Friez, M. J., Lovell, C. R. (2002). Formyltetrahydrofolate Synthetase Sequences from Salt Marsh Plant Roots Reveal a Diversity of Acetogenic Bacteria and Other Bacterial Functional Groups. Appl. Environ. Microbiol. 69: 693-696 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»