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Applied and Environmental Microbiology, April 2001, p. 1412-1417, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1412-1417.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Long-Range 1H-15N Heteronuclear Shift Correlation at Natural Abundance: a Tool To Study Benzothiazole Biodegradation by Two Rhodococcus Strains

Pascale Besse,1 Bruno Combourieu,1 Gaëlle Boyse,1 Martine Sancelme,1 Heleen De Wever,2 and Anne-Marie Delort1,*

Laboratoire de Synthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504 du CNRS, Université Blaise Pascal, 63177 Aubière Cedex, France,1 and Laboratory for Soil Fertility and Soil Biology, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium2

Received 24 October 2000/Accepted 8 January 2001

The biodegradation of benzothiazole and 2-hydroxybenzothiazole by two strains of Rhodococcus was monitored by reversed phase high-pressure liquid chromatography and by 1H nuclear magnetic resonance (NMR). Both xenobiotics were biotransformed into a hydroxylated derivative of 2-hydroxybenzothiazole by these two strains. The chemical structure of this metabolite was determined by a new NMR methodology: long-range 1H-15N heteronuclear shift correlation without any previous 15N enrichment of the compound. This powerful NMR tool allowed us to assign the metabolite structure to 2,6-dihydroxybenzothiazole.

* Corresponding author. Mailing address: Laboratoire de Synthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504 du CNRS, Université Blaise Pascal, 63177 Aubière Cedex, France. Phone: 33-4-73-40-77-14. Fax: 33-4-73-40-77-17. E-mail: amdelort{at}chimtp.univ-bpclermont.fr.

Applied and Environmental Microbiology, April 2001, p. 1412-1417, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1412-1417.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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