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Applied and Environmental Microbiology, April 2001, p. 1429-1436, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1429-1436.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Use of Pulsed-Field Gel Electrophoresis and Flagellin Gene Typing in Identifying Clonal Groups of Campylobacter jejuni and Campylobacter coli in Farm and Clinical Environments

Collette Fitzgerald,* Karen Stanley,dagger Sarah Andrew,Dagger and Keith Jones

Department of Biological Sciences, Lancaster University, Lancaster LA1 4YQ, United Kingdom

Received 30 June 2000/Accepted 15 January 2001

Although campylobacters have been isolated from a wide range of animal hosts, the association between campylobacters isolated from humans and animals in the farm environment is unclear. We used flagellin gene typing and pulsed-field gel electrophoresis (PFGE) to investigate the genetic diversity among isolates from animals (cattle, sheep, and turkey) in farm environments and sporadic cases of campylobacteriosis in the same geographical area. Forty-eight combined fla types were seen among the 315 Campylobacter isolates studied. Six were found in isolates from all four hosts and represented 50% of the total number of isolates. Seventy-one different SmaI PFGE macrorestriction profiles (mrps) were observed, with 86% of isolates assigned to one of 29 different mrps. Fifty-seven isolates from diverse hosts, times, and sources had an identical SmaI mrp and combined fla type. Conversely, a number of genotypes were unique to a particular host. We provide molecular evidence which suggests a link between campylobacters in the farm environment with those causing disease in the community.

* Corresponding author. Present address: Foodborne and Diarrheal Diseases Branch, Mailstop CO3, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333. Phone: (404) 639-0838. Fax: (404) 639-3333. E-mail: chf3{at}cdc.gov.

dagger Present address: Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, Scotland.

Dagger Present address: University College Chester, Parkgate Rd., Chester CH1 4BJ, United Kingdom.

Applied and Environmental Microbiology, April 2001, p. 1429-1436, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1429-1436.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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