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Applied and Environmental Microbiology, April 2001, p. 1484-1489, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1484-1489.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Seasonal Variation and Indirect Monitoring of Microcystin Concentrations in Daechung Reservoir, Korea

Hee-Mock Oh,* Seog June Lee, Jee-Hwan Kim, Hee-Sik Kim, and Byung-Dae Yoon

Environmental Bioresources Laboratory, Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon 305-600, Korea

Received 4 October 2000/Accepted 24 January 2001

Physicochemical and biological water quality, including the microcystin concentration, was investigated from spring to autumn 1999 in the Daechung Reservoir, Korea. The dominant genus in the cyanobacterial blooming season was Microcystis. The microcystin concentration in particulate form increased dramatically from August up to a level of 200 ng liter-1 in early October and thereafter tended to decrease. The microcystin concentration in dissolved form was about 28% of that of the particulate form. The microcystins detected using a protein phosphatase (PP) inhibition assay were highly correlated with those microcystins detected by a high-performance liquid chromatograph (r = 0.973; P < 0.01). Therefore, the effectiveness of a PP inhibition assay for microcystin detection in a high number of water samples was confirmed as easy, quick, and convenient. The microcystin concentration was highly correlated with the phytoplankton number (r = 0.650; P < 0.01) and chlorophyll-a concentration (r = 0.591; P < 0.01). When the microcystin concentration exceeded about 100 ng liter-1, the ratio of particulate to dissolved total nitrogen (TN) or total phosphorus (TP) converged at a value of 0.6. Furthermore, the microcystin concentration was lower than 50 ng liter-1 at a particulate N/P ratio below 8, whereas the microcystin concentration varied quite substantially from 50 to 240 ng liter-1 at a particulate N/P ratio of >8. Therefore, it seems that the microcystin concentration in water can be estimated and indirectly monitored by analyzing the following: the phytoplankton number and chlorophyll-a concentration, the ratio of the particulate and the dissolved forms of N and P, and the particulate N/P ratio when the dominant genus is toxigenic Microcystis.

* Corresponding author. Mailing address: Environmental Bioresources Laboratory, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yusong, Taejon 305-600, Korea. Phone: 82-42-860-4321. Fax: 82-42-860-4598. E-mail: heemock{at}mail.kribb.re.kr.

Applied and Environmental Microbiology, April 2001, p. 1484-1489, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1484-1489.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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