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Applied and Environmental Microbiology, April 2001, p. 1503-1507, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1503-1507.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of Fecal Escherichia coli from Humans and Animals by Ribotyping

C. Andrew Carson,1,* Brian L. Shear,1 Mark R. Ellersieck,2 and Amha Asfaw2

Department of Veterinary Pathobiology1 and Agricultural Experiment Station Statisticians,2 University of Missouri, Columbia, Missouri 65211

Received 16 August 2000/Accepted 16 January 2001

Fecal pollution of water resources is an environmental problem of increasing importance. Identification of individual host sources of fecal Escherichia coli, such as humans, pets, production animals, and wild animals, is prerequisite to formulation of remediation plans. Ribotyping has been used to distinguish fecal E. coli of human origin from pooled fecal E. coli isolates of nonhuman origin. We have extended application of this technique to distinguishing fecal E. coli ribotype patterns from human and seven individual nonhuman hosts. Classification accuracy was best when the analysis was limited to three host sources. Application of this technique to identification of host sources of fecal coliforms in water could assist in formulation of pollution reduction plans.

* Corresponding author. Mailing address: Department of Veterinary Pathobiology, 201 Connaway Hall, University of Missouri, Columbia, MO 65211. Phone: (573) 884-7640. Fax: (573) 884-0521. E-mail: carsonc{at}missouri.edu.

Applied and Environmental Microbiology, April 2001, p. 1503-1507, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1503-1507.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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