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Applied and Environmental Microbiology, April 2001, p. 1508-1516, Vol. 67, No. 4
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.4.1508-1516.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Map of the IncP1
Plasmid pTSA Encoding the
Widespread Genes (tsa) for
p-Toluenesulfonate Degradation in Comamonas
testosteroni T-2
Tewes
Tralau,
Alasdair M.
Cook, and
Juergen
Ruff*
Department of Biology, The University of
Konstanz, D-78457 Konstanz, Germany
Received 25 September 2000/Accepted 4 January 2001
The catabolic IncP1
plasmid pTSA from Comamonas
testosteroni T-2 was mapped by subtractive analysis of
restriction digests, by sequencing outwards from the tsa
operon (toluenesulfonate degradation), and by generating
overlapping, long-distance-PCR amplification products. The plasmid was
estimated to comprise 72 ± 4 kb. The tsa region
was found to be a composite transposon flanked by two IS1071 elements. A cryptic tsa operon was
also present in the tsa transposon. Those backbone genes
and regions which we sequenced were in the same order as the
corresponding genes in resistance plasmid R751, and identities of about
99% were observed. Enrichment cultures with samples from four
continents were done to obtain organisms able to utilize
p-toluenesulfonate as the sole source of carbon and
energy for aerobic growth. Most (15) of the 16 cultures (13 of them
isolates) were obtained from contaminated sites and were attributed to
three metabolic groups, depending on their metabolism of
p-toluenesulfonate. The largest group contained the
tsa transposon, usually (six of seven isolates) with
negligible differences in sequence from strain T-2.
*
Corresponding author. Mailing address: Department of
Biology, Universitätsstr. 10, The University, D-78457
Konstanz, Germany. Phone: (49) 7531 88 2100. Fax: (49) 7531 88 2966. E-mail: juergen.ruff{at}uni-konstanz.de.
Applied and Environmental Microbiology, April 2001, p. 1508-1516, Vol. 67, No. 4
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.4.1508-1516.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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