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Applied and Environmental Microbiology, April 2001, p. 1508-1516, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1508-1516.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Map of the IncP1beta Plasmid pTSA Encoding the Widespread Genes (tsa) for p-Toluenesulfonate Degradation in Comamonas testosteroni T-2

Tewes Tralau, Alasdair M. Cook, and Juergen Ruff*

Department of Biology, The University of Konstanz, D-78457 Konstanz, Germany

Received 25 September 2000/Accepted 4 January 2001

The catabolic IncP1beta plasmid pTSA from Comamonas testosteroni T-2 was mapped by subtractive analysis of restriction digests, by sequencing outwards from the tsa operon (toluenesulfonate degradation), and by generating overlapping, long-distance-PCR amplification products. The plasmid was estimated to comprise 72 ± 4 kb. The tsa region was found to be a composite transposon flanked by two IS1071 elements. A cryptic tsa operon was also present in the tsa transposon. Those backbone genes and regions which we sequenced were in the same order as the corresponding genes in resistance plasmid R751, and identities of about 99% were observed. Enrichment cultures with samples from four continents were done to obtain organisms able to utilize p-toluenesulfonate as the sole source of carbon and energy for aerobic growth. Most (15) of the 16 cultures (13 of them isolates) were obtained from contaminated sites and were attributed to three metabolic groups, depending on their metabolism of p-toluenesulfonate. The largest group contained the tsa transposon, usually (six of seven isolates) with negligible differences in sequence from strain T-2.

* Corresponding author. Mailing address: Department of Biology, Universitätsstr. 10, The University, D-78457 Konstanz, Germany. Phone: (49) 7531 88 2100. Fax: (49) 7531 88 2966. E-mail: juergen.ruff{at}uni-konstanz.de.

Applied and Environmental Microbiology, April 2001, p. 1508-1516, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1508-1516.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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